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Literature summary for 6.3.2.5 extracted from

  • Yao, J.; Patrone, J.D.; Dotson, G.D.
    Characterization and kinetics of phosphopantothenoylcysteine synthetase from Enterococcus faecalis (2009), Biochemistry, 48, 2799-2806.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
CMP competitive to CTP, mixed type to (R)-4'-phosphopantothenate, uncompetitive to L-Cys Enterococcus faecalis
L-Cys
-
 Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 (R)-4'-phosphopantothenate pH 7.6, 37°C Enterococcus faecalis
0.086
-
 L-Cys pH 7.6, 37°C Enterococcus faecalis
0.156
-
 CTP pH 7.6, 37°C Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28415
-
 2 * 28415, ESI-MS Enterococcus faecalis
60000
-
 gel filtration Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Enterococcus faecalis

Reaction

Reaction Comment Organism Reaction ID
CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine bi uni uni bi ping pong mechanism, with the nucleotide CTP binding first and CMP released last Enterococcus faecalis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-4'-phosphopantothenate + L-Cys 8% of the activity with CTP Enterococcus faecalis (R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate
-
 ?
CTP + (R)-4'-phosphopantothenate + L-Cys
-
 Enterococcus faecalis (R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28415, ESI-MS Enterococcus faecalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
 CTP pH 7.6, 37°C Enterococcus faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.766
-
 L-Cys substrate CTP, pH 7.6, 37°C Enterococcus faecalis