Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.5 extracted from

  • Kupke, T.; Schwarz, W.
    4-Phosphopantetheine biosynthesis in archaea (2006), J. Biol. Chem., 281, 5435-5444.
    View publication on PubMed
Search for more literature for 6.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 (pREP4) cells Methanocaldococcus jannaschii
overexpression in Escherichia coli Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
K310Q very low activity Methanocaldococcus jannaschii
K310Q the mutant has very low activity Methanocaldococcus jannaschii
N217H mutant enzyme has residual activity Methanocaldococcus jannaschii
N217H the mutant has only residual enzyme activity Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + (R)-4'-phosphopantothenate + L-cysteine Methanocaldococcus jannaschii
-
 CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
CTP + (R)-4'-phosphopantothenate + L-cysteine Methanocaldococcus jannaschii DSM 2661
-
 CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58323
-
-
Methanocaldococcus jannaschii DSM 2661 Q58323
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Methanocaldococcus jannaschii
purified as His tag protein Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
 Methanocaldococcus jannaschii CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
CTP + (R)-4'-phosphopantothenate + L-cysteine detection of the 4'-phosphopantothenoyl-CMP intermediate Methanocaldococcus jannaschii CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
 Methanocaldococcus jannaschii DSM 2661 CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
CTP + (R)-4'-phosphopantothenate + L-cysteine detection of the 4'-phosphopantothenoyl-CMP intermediate Methanocaldococcus jannaschii DSM 2661 CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Synonyms

Synonyms Comment Organism
CoaB
-
 Methanocaldococcus jannaschii
Dfp
-
 Methanocaldococcus jannaschii
phosphopantothenate-cysteine ligase
-
 Methanocaldococcus jannaschii
PPCS
-
 Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
CTP
-
 Methanocaldococcus jannaschii