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Literature summary for 6.3.2.43 extracted from

  • Ouchi, T.; Tomita, T.; Horie, A.; Yoshida, A.; Takahashi, K.; Nishida, H.; Lassak, K.; Taka, H.; Mineki, R.; Fujimura, T.; Kosono, S.; Nishiyama, C.; Masui, R.; Kuramitsu, S.; Albers, S.V.; Kuzuyama, T.; Nishiyama, M.
    Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus (2013), Nat. Chem. Biol., 9, 277-283.
    View publication on PubMed
Search for more literature for 6.3.2.43

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfolobus acidocaldarius

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of the AMP-PNP–bound complex of LysX Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
N264G/T265T the mutant shows a substrate preference for glutamate over L-2-aminoadipate contrasting distinctly with the substrate specificity of wild-type enzyme, which shows a distinct preference for L-2-aminoadipate over glutamate Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate Sulfolobus acidocaldarius lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW ADP + phosphate + [LysW]-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate Sulfolobus acidocaldarius DSM 639 lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW ADP + phosphate + [LysW]-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4JAP9
-
-
Sulfolobus acidocaldarius DSM 639 Q4JAP9
-
-
Thermus thermophilus Q5SH23
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SH23
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius ADP + phosphate + [LysW]-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius DSM 639 ADP + phosphate + [LysW]-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate mutant enzyme N264G/T265T shows a substrate preference for glutamate over L-2-aminoadipate contrasting distinctly with the substrate specificity of wild-type enzyme, which shows a distinct preference for L-2-aminoadipate over glutamate Sulfolobus acidocaldarius ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-2-aminoadipate mutant enzyme N264G/T265T shows a substrate preference for glutamate over L-2-aminoadipate contrasting distinctly with the substrate specificity of wild-type enzyme, which shows a distinct preference for L-2-aminoadipate over glutamate Sulfolobus acidocaldarius DSM 639 ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
LysX
-
 Sulfolobus acidocaldarius
Saci_0754 locus name Sulfolobus acidocaldarius
TtLysX
-
 Thermus thermophilus

General Information

General Information Comment Organism
malfunction no growth of deletion mutant without L-lysine. Growth is possible with lysine supplementation. A 0.05-mM concentration of lysine is most effective to support the growth of the deletion mutant, but a higher concentration of lysine decreases the growth rate Sulfolobus acidocaldarius
physiological function lysine biosynthesis. Protection of the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius