Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | stimulates | Mus musculus | |
dithiothreitol | the enzyme is markedly stimulated by dithiothreitol, which increases the activity by about 5fold | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expressed in bacteria or HEK293T cells | Mus musculus |
expressed in Escherichia coli | Mus musculus |
expressed in Escherichia coli BL21(DE3)pLysS cells and in HEK-293T cells | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
ATP | pH 8.0, 30°C | Mus musculus | |
0.065 | - |
ATP | pH 8.0, 30°C | Mus musculus | |
0.065 | - |
ATP | with 5 mM MgATP, at pH 8.0 and 37°C | Mus musculus | |
0.73 | - |
L-glutamate | pH 8.0, 30°C | Mus musculus | |
0.87 | - |
citrate | at pH 8.0 and 37°C | Mus musculus | |
0.88 | - |
L-glutamate | pH 8.0, 30°C | Mus musculus | |
0.88 | - |
L-glutamate | at pH 8.0 and 37°C | Mus musculus | |
1.48 | - |
N-acetyl-L-aspartate | pH 8.0, 30°C | Mus musculus | |
1.48 | - |
N-acetyl-L-aspartate | at pH 8.0 and 37°C | Mus musculus | |
4.59 | - |
N-acetyl-L-aspartate | pH 8.0, 30°C | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Mus musculus |
43000 | - |
x * 43000, SDS-PAGE | Mus musculus |
43000 | - |
x * 43000, contains at least six subunits, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-aspartate + L-glutamate | Mus musculus | - |
ADP + phosphate + N-acetyl-L-aspartyl-L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q6PFX8 | - |
- |
Mus musculus | Q80WS1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, and S-200 gel filtration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
central nervous system | - |
Mus musculus | - |
dorsal root ganglion | - |
Mus musculus | - |
placenta | - |
Mus musculus | - |
spinal cord | - |
Mus musculus | - |
testis | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.09 | - |
pH 8.0, 30°C, formation of N-acetyl-L-aspartyl-L-glutamate | Mus musculus |
1.4 | - |
pH 8.0, 30°C, formation of N-acetyl-L-aspartyl-L-glutamate | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-L-aspartate + citrate | - |
Mus musculus | ? | - |
? | |
ATP + N-acetyl-L-aspartate + L-glutamate | - |
Mus musculus | ADP + phosphate + N-acetyl-L-aspartyl-L-glutamate | - |
? | |
ATP + N-acetyl-L-aspartate + L-glutamate | isoform RIMKLA also catalyses the synthesis of beta-citryl-L-glutamate with an activity that is 75fold lower than its N-acetyl-L-aspartyl-L-glutamate synthase activity | Mus musculus | ADP + phosphate + N-acetyl-L-aspartyl-L-glutamate | - |
? | |
ATP + N-acetyl-L-aspartate + L-glutamate | isoform RIMKLB catalyses the synthesis of beta-citryl-L-glutamate and N-acetyl-L-aspartyl-L-glutamate at nearly equal rates, cf. EC 6.3.1.17, beta-citrylglutamate synthase | Mus musculus | ADP + phosphate + N-acetyl-L-aspartyl-L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Mus musculus |
? | x * 43000, SDS-PAGE | Mus musculus |
? | x * 43000, contains at least six subunits, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
N-acetylaspartylglutamate synthetase | - |
Mus musculus |
NAAG synthetase | - |
Mus musculus |
NAAGS | - |
Mus musculus |
RIMKLA | - |
Mus musculus |
RIMKLA | gene name, ambiguous | Mus musculus |
RIMKLB | gene name, ambiguous | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
N-acetyl-L-aspartate | pH 8.0, 30°C | Mus musculus | |
2.6 | - |
N-acetyl-L-aspartate | at pH 8.0 and 37°C | Mus musculus | |
3 | - |
N-acetyl-L-aspartate | pH 8.0, 30°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mus musculus |
8 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |