Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for drug development to combat against Mycobacterium tuberculosis (Mtb) | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
gene ddlA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-cycloserine | a competitive inhibitor | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Mycobacterium tuberculosis | |
0.0503 | - |
ATP | pH 8.0, 37°C, recombinant His-tagged enzyme | Mycobacterium tuberculosis | |
1.011 | - |
D-alanine | pH 8.0, 37°C, recombinant His-tagged enzyme | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 D-alanine | Mycobacterium tuberculosis | - |
ADP + phosphate + D-alanyl-D-alanine | - |
? | |
ATP + 2 D-alanine | Mycobacterium tuberculosis ATCC 25618 / H37Rv | - |
ADP + phosphate + D-alanyl-D-alanine | - |
? | |
additional information | Mycobacterium tuberculosis | putative protein interaction partners of enzyme DdlA from Mycobacterium tuberculosis, overview | ? | - |
? | |
additional information | Mycobacterium tuberculosis ATCC 25618 / H37Rv | putative protein interaction partners of enzyme DdlA from Mycobacterium tuberculosis, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WP31 | - |
- |
Mycobacterium tuberculosis ATCC 25618 / H37Rv | P9WP31 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine | first, the ATP-dependent dimerization reactions catalyzed by DdlA proceed through the phosphorylation of the D-alanine at the carboxyl group by the gamma-phosphate of ATP to produce an acylphosphate intermediate. Then, a tetrahedral intermediate is formed by the nucleophilic attacking of the amino nitrogen of the second D-alanine on the acylphosphate intermediate. Finally, a phosphate group is released from the tetrahedral intermediate to generate the final product of D-alanyl-D-alanine | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 D-alanine | - |
Mycobacterium tuberculosis | ADP + phosphate + D-alanyl-D-alanine | - |
? | |
ATP + 2 D-alanine | DdlA catalyzes the dimerization of two D-alanine molecules and typically couples ATP hydrolysis to provide a thermodynamic driving force and exhibits a cleavage of ATP to ADP and phosphate. D-Alanine is completely converted into D-alanyl-D-alanine, so no remaining D-alanine is detected | Mycobacterium tuberculosis | ADP + phosphate + D-alanyl-D-alanine | - |
? | |
ATP + 2 D-alanine | - |
Mycobacterium tuberculosis ATCC 25618 / H37Rv | ADP + phosphate + D-alanyl-D-alanine | - |
? | |
ATP + 2 D-alanine | DdlA catalyzes the dimerization of two D-alanine molecules and typically couples ATP hydrolysis to provide a thermodynamic driving force and exhibits a cleavage of ATP to ADP and phosphate. D-Alanine is completely converted into D-alanyl-D-alanine, so no remaining D-alanine is detected | Mycobacterium tuberculosis ATCC 25618 / H37Rv | ADP + phosphate + D-alanyl-D-alanine | - |
? | |
additional information | putative protein interaction partners of enzyme DdlA from Mycobacterium tuberculosis, overview | Mycobacterium tuberculosis | ? | - |
? | |
additional information | putative protein interaction partners of enzyme DdlA from Mycobacterium tuberculosis, overview | Mycobacterium tuberculosis ATCC 25618 / H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39680, sequence calculation, x * 40000, about, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
DdlA | - |
Mycobacterium tuberculosis |
Rv2981c | - |
Mycobacterium tuberculosis |
Tb-DdlA | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00073 | - |
ATP | pH 8.0, 37°C, recombinant His-tagged enzyme | Mycobacterium tuberculosis | |
0.00073 | - |
D-alanine | pH 8.0, 37°C, recombinant His-tagged enzyme | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | two different metabolic pathways, of DdlA and LysA, respectively, leading to peptidoglycan biosynthesis, overview | Mycobacterium tuberculosis |
physiological function | D-alanine-D-alanine ligase (DdlA) supplies D-alanyl-D-alanine as a substrate for peptidoglycan crosslinking by catalyzing the dimerization of two D-alanines, the enzyme is involved in the pentapeptide biosynthesis of peptidoglycan | Mycobacterium tuberculosis |