Protein Variants | Comment | Organism |
---|---|---|
S137A/Y207F | higher D-alanyl-D-lactate and lower D-alanyl-D-alanine synthase activity than mutant Y207F. Broad substrate specificity toward D-amino acids, accepts D-lactate and D,L-isoleucine as substrate | Thermotoga maritima |
S137F/Y207F | about 20% of wild-type activity. Accepts D-lactate as substrate | Thermotoga maritima |
S137G/Y207F | about 200% of wild-type activity. Accepts D-lactate as substrate | Thermotoga maritima |
S137T/Y207F | about 40% of wild-type activity. Does not accept D-lactate as substrate | Thermotoga maritima |
Y201F | increase in activity compared to wild-type, mutant is not able to synthesize D-alanyl-D-lactate | Thermotoga maritima |
Y207F | mutant is able to synthesize D-alanyl-D-lactate | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Thermotoga maritima ATCC 43589 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.19 | - |
mutant S137F/Y207F, substrates D-Ala + D-lactate, 60°C, pH 7.5 | Thermotoga maritima |
0.39 | - |
mutant Y207F, substrates D-Ala + D-lactate, 60°C, pH 7.5 | Thermotoga maritima |
0.42 | - |
mutant S137A/Y207F, substrates D-Ala + D-lactate, 60°C, pH 7.5 | Thermotoga maritima |
0.95 | - |
mutant S137F/Y207F, substrate D-Ala, 60°C, pH 7.5 | Thermotoga maritima |
1.2 | - |
mutant S137G/Y207F, substrates D-Ala + D-lactate, 60°C, pH 7.5 | Thermotoga maritima |
1.75 | - |
mutant S137T/Y207F, substrate D-Ala, 60°C, pH 7.5 | Thermotoga maritima |
4.22 | - |
wild-type, substrate D-Ala, 60°C, pH 7.5 | Thermotoga maritima |
6.489 | - |
mutant S137A/Y207F, substrate D-Ala, 60°C, pH 7.5 | Thermotoga maritima |
9.66 | - |
mutant S137G/Y207F, substrate D-Ala, 60°C, pH 7.5 | Thermotoga maritima |
17.22 | - |
mutant Y207F, substrate D-Ala, 60°C, pH 7.5 | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-Ala + D-Ala | - |
Thermotoga maritima | ADP + phosphate + D-Ala-D-Ala | - |
? | |
ATP + D-Ala + D-Ala | - |
Thermotoga maritima ATCC 43589 | ADP + phosphate + D-Ala-D-Ala | - |
? | |
ATP + D-Ala + D-lactate | D-lactate is not a substrate for wild-type, but for mutants S137A/Y207F, S137F/Y207F, S137T/Y207F, S137G/Y207F, Y207F | Thermotoga maritima | ADP + phosphate + D-Ala-D-lactate | - |
? | |
ATP + D-Ala + D-lactate | D-lactate is not a substrate for wild-type, but for mutants S137A/Y207F, S137F/Y207F, S137T/Y207F, S137G/Y207F, Y207F | Thermotoga maritima ATCC 43589 | ADP + phosphate + D-Ala-D-lactate | - |
? |