Literature summary for 6.3.2.4 extracted from

Ellsworth, B.A.; Tom, N.J.; Bartlett, P.A.
Synthesis and evaluation of inhibitors of bacterial D-alanine:D-alanine ligases (1996), Chem. Biol., 3, 37-44.

Search for more literature for 6.3.2.4

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	large reduction in binding affinity of the VanA mutant enzyme for all the phosphorus-containing analogs has significant implications for the design of inhibitors specific for this enzyme	Enterococcus faecium
Phosphinates	weak inhibition of ligase B of E. coli and Van A from Enterococcus faecium, strong inhibition of ligase A from E. coli	Enterococcus faecium
Phosphinates	weak inhibition of ligase B of E. coli and Van A from Enterococcus faecium, strong inhibition of ligase A from E. coli	Escherichia coli
Phosphonate dipeptide analogs	weak inhibition of ligase B from E. coli and Van A from Enterococcus faecium	Enterococcus faecium
Phosphonate dipeptide analogs	weak inhibition of ligase B from E. coli and Van A from Enterococcus faecium	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0033	-	D-Ala	N-terminal Ala, ligase B	Escherichia coli
0.0057	-	D-Ala	C-terminal Ala, ligase A	Escherichia coli
0.55	-	D-Ala	D-Ala, C-terminal, ligase A	Escherichia coli
1.2	-	D-Ala	C-terminal Ala, ligase B	Escherichia coli
3.4	-	D-Ala	N-terminal Ala	Enterococcus faecium
38	-	D-Ala	-	Escherichia coli
38	-	D-Ala	C-terminal Ala	Enterococcus faecium

Organism

Organism	UniProt	Comment	Textmining
Enterococcus faecium	-	mutant enzyme that incorporats 2-hydroxy acids at terminal site instead of D-Ala	-
Escherichia coli	-	2 enzyme forms: ligase A and ligase B	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-Ala + D-Ala	-	Escherichia coli	ADP + phosphate + D-Ala-D-Ala	-	?
ATP + D-Ala + D-Ala	-	Enterococcus faecium	ADP + phosphate + D-Ala-D-Ala	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Escherichia coli
additional information	-	additional information	-	Enterococcus faecium

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Release 2023.1 (January 2023)