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Literature summary for 6.3.2.37 extracted from

  • Boniface, A.; Bouhss, A.; Mengin-Lecreulx, D.; Blanot, D.
    The MurE synthetase from Thermotoga maritima is endowed with an unusual D-lysine adding activity (2006), J. Biol. Chem., 281, 15680-15686.
    View publication on PubMed
Search for more literature for 6.3.2.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed as C-terminal His6-tagged enzyme in Escherichia coli DH5alpha cells Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
UDP-N-acetylmuramoyl-L-Ala-D-Glu strong inhibition by excess UDP-N-acetylmuramoyl-L-Ala-D-Glu is observed Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the optimum Mg2+ concentration for L-lysine adding activity is 15 mM Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 SDS-PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, gel filtration Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetylmuramoyl-L-Ala-D-Glu + D-Lys + ATP D-lysine is added to UDP-N-acetylmuramoyl-L-Ala-D-Glu with 25% efficiency relative to L-lysine Thermotoga maritima UDP-N-acetylmuramoyl-L-Ala-D-Glu-D-Lys + ADP + phosphate
-
 ?
UDP-N-acetylmuramoyl-L-Ala-D-Glu + D-Lys + ATP D-lysine is added to UDP-N-acetylmuramoyl-L-Ala-D-Glu with 25% efficiency relative to L-lysine Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 UDP-N-acetylmuramoyl-L-Ala-D-Glu-D-Lys + ADP + phosphate
-
 ?
UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys + ATP
-
 Thermotoga maritima UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + ADP + phosphate
-
 ?
UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid + ATP meso-diaminopimelic acid is added to UDP-N-acetylmuramoyl-L-Ala-D-Glu with 10% efficiency relative to L-lysine Thermotoga maritima UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid + ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
MurE
-
 Thermotoga maritima
MurE synthetase
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
68
-
 temperature optimum for L-lysine adding activity. At 37°C, the activity is about 25% of the optimal value Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
 for L-lysine adding activity Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Thermotoga maritima