Literature summary for 6.3.2.30 extracted from

Kroll, J.; Klinter, S.; Steinbuechel, A.
A novel plasmid addiction system for large-scale production of cyanophycin in Escherichia coli using mineral salts medium (2011), Appl. Microbiol. Biotechnol., 89, 593-604.

Search for more literature for 6.3.2.30

Application

Application	Comment	Organism
synthesis	synthesis of cyanophycin using an anabolism-based media-dependent plasmid addiction system to enhance plasmid stability, and a process based on a modified mineral salts medium yielding a cyanophycin content of 42% w/w at the maximum without the addition of amino acids to the medium. This plasmid addiction system is based on different lysine biosynthesis pathways and consists of a knockout of the chromosomal dapE that disrupts the native succinylase pathway in Escherichia coli and the complementation by the plasmid-encoded artificial aminotransferase pathway mediated by the dapL gene from Synechocystis sp. PCC 6308, which allows the synthesis of the essential lysine precursor L,L-2,6-diaminopimelate. This plasmid also harbors an engineered cyanophycin synthetase gene responsible for cyanophycin production.Cultivation experiments reveal an at least 4.5fold enhanced production of cyanophycin in comparison to control cultivations	Synechocystis sp.

Protein Variants

Protein Variants	Comment	Organism
C595S	enhanced catalytic activity	Synechocystis sp.

Organism

Organism	UniProt	Comment	Textmining
Synechocystis sp.	P56947	cf. EC 6.3.2.29	-
Synechocystis sp. PCC 6308	P56947	cf. EC 6.3.2.29	-

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)