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Literature summary for 6.3.2.30 extracted from

  • Aboulmagd, E.; Oppermann-Sanio, F.B.; Steinbüchel, A.
    Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308 (2000), Arch. Microbiol., 174, 297-306.
    View publication on PubMed
Search for more literature for 6.3.2.30

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli TOP10 cells Synechocystis sp. PCC 6803

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6805
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from Synechocystis and from recombinant Escherichia coli cells Synechocystis sp. PCC 6803

Reaction

Reaction Comment Organism Reaction ID
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by the enzyme's other active centre Synechocystis sp. PCC 6803
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg a small amount of cyanophycin is required as a primer Synechocystis sp. PCC 6803 ADP + phosphate + [L-Asp(4-L-Arg)]n+1 no formation of AMP, [L-Asp(3-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase ?

Synonyms

Synonyms Comment Organism
CphA
-
 Synechocystis sp. PCC 6803
cyanophycin synthetase
-
 Synechocystis sp. PCC 6803