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Literature summary for 6.3.2.3 extracted from

  • Yang, J.; Li, W.; Wang, D.; Wu, H.; Li, Z.; Ye, Q.
    Characterization of bifunctional L-glutathione synthetases from Actinobacillus pleuropneumoniae and Actinobacillus succinogenes for efficient glutathione biosynthesis (2016), Appl. Microbiol. Biotechnol., 100, 6279-6289 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gshF, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Actinobacillus pleuropneumoniae
gene gshF, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Actinobacillus succinogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Actinobacillus pleuropneumoniae
additional information
-
additional information kinetics Actinobacillus succinogenes
0.299
-
ATP pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus pleuropneumoniae
0.356
-
ATP pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus succinogenes
1.13
-
gamma-L-glutamyl-L-cysteine pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus pleuropneumoniae
1.3
-
glycine pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus pleuropneumoniae
1.56
-
gamma-L-glutamyl-L-cysteine pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus succinogenes
2.1
-
glycine pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus succinogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Actinobacillus pleuropneumoniae
Mg2+ required Actinobacillus succinogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + gamma-L-glutamyl-L-cysteine + glycine Actinobacillus pleuropneumoniae
-
ADP + phosphate + glutathione
-
?
ATP + gamma-L-glutamyl-L-cysteine + glycine Actinobacillus succinogenes
-
ADP + phosphate + glutathione
-
?
ATP + gamma-L-glutamyl-L-cysteine + glycine Actinobacillus succinogenes ATCC 55618
-
ADP + phosphate + glutathione
-
?
ATP + gamma-L-glutamyl-L-cysteine + glycine Actinobacillus pleuropneumoniae ATCC 55618
-
ADP + phosphate + glutathione
-
?

Organism

Organism UniProt Comment Textmining
Actinobacillus pleuropneumoniae D4N892
-
-
Actinobacillus pleuropneumoniae ATCC 55618 D4N892
-
-
Actinobacillus succinogenes A6VQP8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Actinobacillus pleuropneumoniae
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Actinobacillus succinogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
GshFAp has a glutathione synthetase catalytic activity with Vmax of 775 U/mg Actinobacillus pleuropneumoniae
additional information
-
GshFAp has a high gamma-GCS catalytic activity with Vmax of 1393 U/mg Actinobacillus succinogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-L-glutamyl-L-cysteine + glycine
-
Actinobacillus pleuropneumoniae ADP + phosphate + glutathione
-
?
ATP + gamma-L-glutamyl-L-cysteine + glycine
-
Actinobacillus succinogenes ADP + phosphate + glutathione
-
?
ATP + gamma-L-glutamyl-L-cysteine + glycine
-
Actinobacillus succinogenes ATCC 55618 ADP + phosphate + glutathione
-
?
ATP + gamma-L-glutamyl-L-cysteine + glycine
-
Actinobacillus pleuropneumoniae ATCC 55618 ADP + phosphate + glutathione
-
?

Synonyms

Synonyms Comment Organism
Asuc_1947
-
Actinobacillus pleuropneumoniae
Asuc_1947
-
Actinobacillus succinogenes
bifunctional L-glutathione synthetase
-
Actinobacillus pleuropneumoniae
bifunctional L-glutathione synthetase
-
Actinobacillus succinogenes
gamma-GCS
-
Actinobacillus pleuropneumoniae
glutathione biosynthesis bifunctional protein GshAB UniProt Actinobacillus pleuropneumoniae
glutathione biosynthesis bifunctional protein GshAB UniProt Actinobacillus succinogenes
gshAB gene name, UniProt Actinobacillus pleuropneumoniae
gshAB gene name, UniProt Actinobacillus succinogenes
GshF
-
Actinobacillus pleuropneumoniae
GshF
-
Actinobacillus succinogenes
GshFAp
-
Actinobacillus pleuropneumoniae
GshFAs
-
Actinobacillus succinogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Actinobacillus pleuropneumoniae
40
-
-
Actinobacillus succinogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Actinobacillus pleuropneumoniae
9
-
-
Actinobacillus succinogenes

Cofactor

Cofactor Comment Organism Structure
ATP
-
Actinobacillus pleuropneumoniae
ATP
-
Actinobacillus succinogenes