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Literature summary for 6.3.2.3 extracted from
- Characterization of Plasmodium berghei glutathione synthetase (2011), Parasitol. Int., 60, 321-323.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 40% residual activity | Plasmodium berghei |  |
| Ba2+ | 27% residual activity | Plasmodium berghei | |
| Cd2+ | complete inhibition | Plasmodium berghei | |
| Chloroquine | 0.1 mM, 24% inhibition | Plasmodium berghei | |
| Co2+ | complete inhibition | Plasmodium berghei | |
| Cu2+ | 15% residual activity | Plasmodium berghei | |
| Fe3+ | 24% residual activity | Plasmodium berghei | |
| glutathione | noncompetitive | Plasmodium berghei | |
| Hg2+ | complete inhibition | Plasmodium berghei | |
| mefloquine | 0.1 mM, 21% inhibition | Plasmodium berghei | |
| additional information | no significant inhibition: amoxycillin, gentamycin, streptomycin, kanamycin, tetracycline, penicillin, artesunate, aablaquine and primaquine | Plasmodium berghei | |
| Ni2+ | complete inhibition | Plasmodium berghei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
ATP | pH 8.2, temperature not specified in the publication | Plasmodium berghei | |
| 0.43 | - |
Gly | pH 8.2, 37°C | Plasmodium berghei | |
| 8.3 | - |
gamma-L-Glu-L-Cys | pH 8.2, 37°C | Plasmodium berghei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Plasmodium berghei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium berghei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from Plasmodium berghei infected mouse erythrocytes | Plasmodium berghei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29.95 | - |
pH 8.2, 37°C | Plasmodium berghei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-L-Glu-L-Cys + Gly | - |
Plasmodium berghei | ADP + phosphate + glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 70000, SDS-PAGE | Plasmodium berghei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Plasmodium berghei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, no residual activity | Plasmodium berghei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Plasmodium berghei |
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