Protein Variants | Comment | Organism |
---|---|---|
G369V | mutation in G-loop glycine triad, about 0.7% of wild-type activity. Mutation decreases ligand binding and prevent active site closure and protection | Homo sapiens |
G370V | mutation in G-loop glycine triad, about 0.3% of wild-type activity. Mutation decreases ligand binding and prevent active site closure and protection | Homo sapiens |
G371V | mutation in G-loop glycine triad, about 13% of wild-type activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
ATP | wild-type, 37°C, pH not specified in the publication | Homo sapiens | |
0.1 | - |
gamma-L-Glu-aminobutanoate | mutant G371V, 37°C, pH not specified in the publication | Homo sapiens | |
0.12 | - |
ATP | mutant G371V, 37°C, pH not specified in the publication | Homo sapiens | |
0.44 | - |
ATP | mutant G369V, 37°C, pH not specified in the publication | Homo sapiens | |
0.53 | - |
Gly | wild-type, 37°C, pH not specified in the publication | Homo sapiens | |
0.89 | - |
gamma-L-Glu-aminobutanoate | mutant G369V, 37°C, pH not specified in the publication | Homo sapiens | |
1.26 | - |
gamma-L-Glu-aminobutanoate | wild-type, 37°C, pH not specified in the publication | Homo sapiens | |
2.52 | - |
Gly | mutant G371V, 37°C, pH not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-L-Glu-aminobutanoate + Gly | - |
Homo sapiens | ADP + phosphate + ophthalmic acid | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
Gly | mutant G370V, 37°C, pH not specified in the publication | Homo sapiens | |
0.133 | - |
Gly | mutant G369V, 37°C, pH not specified in the publication | Homo sapiens | |
2.36 | - |
Gly | mutant G371V, 37°C, pH not specified in the publication | Homo sapiens | |
18.8 | - |
Gly | wild-type, 37°C, pH not specified in the publication | Homo sapiens |