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Literature summary for 6.3.2.3 extracted from

  • Dinescu, A.; Brown, T.R.; Barelier, S.; Cundari, T.R.; Anderson, M.E.
    The role of the glycine triad in human glutathione synthetase (2010), Biochem. Biophys. Res. Commun., 400, 511-516.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
G369V mutation in G-loop glycine triad, about 0.7% of wild-type activity. Mutation decreases ligand binding and prevent active site closure and protection Homo sapiens
G370V mutation in G-loop glycine triad, about 0.3% of wild-type activity. Mutation decreases ligand binding and prevent active site closure and protection Homo sapiens
G371V mutation in G-loop glycine triad, about 13% of wild-type activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
ATP wild-type, 37°C, pH not specified in the publication Homo sapiens
0.1
-
gamma-L-Glu-aminobutanoate mutant G371V, 37°C, pH not specified in the publication Homo sapiens
0.12
-
ATP mutant G371V, 37°C, pH not specified in the publication Homo sapiens
0.44
-
ATP mutant G369V, 37°C, pH not specified in the publication Homo sapiens
0.53
-
Gly wild-type, 37°C, pH not specified in the publication Homo sapiens
0.89
-
gamma-L-Glu-aminobutanoate mutant G369V, 37°C, pH not specified in the publication Homo sapiens
1.26
-
gamma-L-Glu-aminobutanoate wild-type, 37°C, pH not specified in the publication Homo sapiens
2.52
-
Gly mutant G371V, 37°C, pH not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-L-Glu-aminobutanoate + Gly
-
Homo sapiens ADP + phosphate + ophthalmic acid
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.052
-
Gly mutant G370V, 37°C, pH not specified in the publication Homo sapiens
0.133
-
Gly mutant G369V, 37°C, pH not specified in the publication Homo sapiens
2.36
-
Gly mutant G371V, 37°C, pH not specified in the publication Homo sapiens
18.8
-
Gly wild-type, 37°C, pH not specified in the publication Homo sapiens