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Literature summary for 6.3.2.3 extracted from

  • Pasternak, M.; Lim, B.; Wirtz, M.; Hell, R.; Cobbett, C.S.; Meyer, A.J.
    Restricting glutathione biosynthesis to the cytosol is sufficient for normal plant development (2008), Plant J., 53, 999-1012.
    View publication on PubMed
Search for more literature for 6.3.2.3

Cloned(Commentary)

Cloned (Comment) Organism
gene gsh2, physical mapping and genotyping, expression analysis, overview Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of gsh2 insertion mutants, the mutants have a seedling lethal phenotype in contrast to the embryo lethal phenotype of gamma-glutamate cysteine ligase, gsh1, EC 6.3.2.2, null mutants, overview. The mutants show hyperaccumulation of gamma-glutamylcysteine to levels 5000-fold that in the wild-type and 200fold wild-type levels of GSH, phenotype, overview. Complementation of gsh2 mutants with the cytosol-specific GSH2 give rise to phenotypically wild-type transgenic plants Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-
cytosol
-
 Arabidopsis thaliana 5829
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + gamma-L-glutamyl-L-cysteine + glycine Arabidopsis thaliana GSH homeostasis in plants is essential for cellular redox control and efficient responses to abiotic and biotic stress. Compartmentation of the GSH biosynthetic pathway is a unique feature of plants, restricting glutathione biosynthesis to the cytosol is sufficient for normal plant development, overview ADP + phosphate + glutathione
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P46416 gene gsh2
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Arabidopsis thaliana
-
leaf
-
 Arabidopsis thaliana
-
seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-L-glutamyl-L-cysteine + glycine
-
 Arabidopsis thaliana ADP + phosphate + glutathione
-
 ?
ATP + gamma-L-glutamyl-L-cysteine + glycine GSH homeostasis in plants is essential for cellular redox control and efficient responses to abiotic and biotic stress. Compartmentation of the GSH biosynthetic pathway is a unique feature of plants, restricting glutathione biosynthesis to the cytosol is sufficient for normal plant development, overview Arabidopsis thaliana ADP + phosphate + glutathione
-
 ?

Synonyms

Synonyms Comment Organism
Glutathione synthetase
-
 Arabidopsis thaliana
GSH2
-
 Arabidopsis thaliana