Cloned (Comment) | Organism |
---|---|
gene cphA, subcloning and expression of His-tagged wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3) | Nostoc ellipsosporum |
Protein Variants | Comment | Organism |
---|---|---|
E856A | site-directed mutagenesis, inactive mutant | Nostoc ellipsosporum |
E856V | site-directed mutagenesis, inactive mutant | Nostoc ellipsosporum |
additional information | construction of truncation mutant CphANE1?C45, which lacks up to 45 amino acids at its C-terminus, the mutant retains full enzymatic activity and produced polymers, however, the removal of one additional amino acid, Glu856, results in complete inactivation of CphANE1?C46, phenotype, overview. Removal of the sequence Leu867-Leu870 results in dramatically decreased thermostability | Nostoc ellipsosporum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Nostoc ellipsosporum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
210000 | - |
gel filtration, recombinant wild-type enzyme | Nostoc ellipsosporum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n + L-Asp | Nostoc ellipsosporum | - |
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp | - |
? | |
ATP + [L-Asp(4-L-Arg)]n + L-Asp | Nostoc ellipsosporum NE1 | - |
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc ellipsosporum | - |
- |
- |
Nostoc ellipsosporum NE1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Nostoc ellipsosporum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n + L-Asp | - |
Nostoc ellipsosporum | ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp | - |
? | |
ATP + [L-Asp(4-L-Arg)]n + L-Asp | role of the C-terminal region of CphANE1, Glu856 is critical for CphANE1 catalytic activity, overview | Nostoc ellipsosporum | ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp | - |
? | |
ATP + [L-Asp(4-L-Arg)]n + L-Asp | - |
Nostoc ellipsosporum NE1 | ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp | - |
? | |
additional information | the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29 | Nostoc ellipsosporum | ? | - |
? | |
additional information | the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29 | Nostoc ellipsosporum NE1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | wild-type and truncation mutant CphANE1?C45 | Nostoc ellipsosporum |
Synonyms | Comment | Organism |
---|---|---|
CphA | - |
Nostoc ellipsosporum |
cyanophycin synthetase | - |
Nostoc ellipsosporum |
More | cf. EC 6.3.2.30 | Nostoc ellipsosporum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the predicted alpha-helical region that ranges from Val858 to Leu870 is important for the thermostability of the enzyme | Nostoc ellipsosporum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Nostoc ellipsosporum |