Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.25 extracted from

  • Kalisz, H.M.; Erck, C.; Plessmann, U.; Wehland, J.
    Incorporation of nitrotyrosine into alpha-tubulin by recombinant mammalian tubulin-tyrosine ligase (2000), Biochim. Biophys. Acta, 1481, 131-138.
    View publication on PubMed
Search for more literature for 6.3.2.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
3-nitro-L-tyrosine 50% inhibition of tyrosine incorporation at 50fold higher concentrations of nitrotyrosine Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0068
-
 L-tyrosine 37°C Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE, immunoblot Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + detyrosinated alpha-tubulin + L-tyrosine Sus scrofa
-
 alpha-tubulin + ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GST-TTL fusion protein, glutathione-sepharose affinity chromatography Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
-70°C, 25 mM K+-morpholinoethanesulfonic acid, pH 6.8, 150 mM KCl, 12.5 MgCl2, 2.5 mM ATP, 1 mM dithiothreitol, 15% glycerol, at least 4 months, no loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + detyrosinated alpha-tubulin + 3-nitro-L-tyrosine 35fold lower affinity for nitrotyrosine than for L-tyrosine, nitrotyrosine is incoorporated into the C-terminus Sus scrofa nitrotyrosinated alpha-tubulin + ADP + phosphate
-
 ?
ATP + detyrosinated alpha-tubulin + L-tyrosine
-
 Sus scrofa alpha-tubulin + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE, immunoblot Sus scrofa

Synonyms

Synonyms Comment Organism
TTL
-
 Sus scrofa
tubuline-tyrosine ligase
-
 Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.24
-
 3-nitro-L-tyrosine 37°C Sus scrofa