KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0254 | - |
L-Tyr | - |
Sus scrofa | |
0.186 | - |
L-Dopa | - |
Sus scrofa | |
0.432 | - |
L-Phe | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
glycerol density gradient centrifugation. The MW of the ligase preincubated with excess phosphocellulose-tubulin is 150000 | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.324 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-70°C, in presence of 40% or 20% glycerol the enzyme can be stored for at least 6 months at a protein concentration of 0.02 mg/ml | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + detyrosinated alpha-tubulin + L-DOPA | - |
Sus scrofa | ? | - |
? | |
ATP + detyrosinated alpha-tubulin + L-Phe | - |
Sus scrofa | ? | - |
? | |
ATP + detyrosinated alpha-tubulin + tyramine | very low activity | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Sus scrofa |