BRENDA - Enzyme Database show
show all sequences of 6.3.2.23

Glutathione and homoglutathione play a critical role in the nodulation process of Medicago truncatula

Frendo, P.; Harrison, J.; Norman, C.; Hernandez Jimenez, M.J.; Van de Sype, G.; Gilabert, A.; Puppo, A.; Mol. Plant Microbe Interact. 18, 254-259 (2005)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
Medicago truncatula
deficiency on GSH and hGSH synthesis inhibits the formation of the root nodule
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Medicago truncatula
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Medicago truncatula
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
deficiency on GSH and hGSH synthesis inhibits the formation of the root nodule
662922
Medicago truncatula
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
Medicago truncatula
deficiency on GSH and hGSH synthesis inhibits the formation of the root nodule
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Medicago truncatula
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
deficiency on GSH and hGSH synthesis inhibits the formation of the root nodule
662922
Medicago truncatula
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
-
-
-
?
Other publictions for EC 6.3.2.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728056
Clemente
Thiol synthetases of legumes: ...
Lotus japonicus, Medicago sativa, Phaseolus vulgaris
J. Exp. Bot.
63
3923-3934
2012
6
-
3
-
-
-
-
-
12
3
3
3
-
5
-
-
3
-
-
6
-
-
3
3
3
-
-
-
3
-
-
3
-
-
-
6
-
3
3
-
-
-
-
-
-
-
12
3
3
3
-
-
-
3
-
6
-
-
3
3
3
-
-
-
3
-
-
-
2
2
2
2
-
-
728585
Dworeck
Site directed mutagenesis of S ...
Medicago truncatula
PLoS ONE
7
e46580
2012
-
-
-
-
1
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
705375
Cruz de Carvalho
Homoglutathione synthetase and ...
Vigna unguiculata
J. Plant Physiol.
167
480-487
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
706219
Galant
Structural basis for evolution ...
Glycine max
Plant Cell
21
3450-3458
2009
-
-
1
1
3
-
-
10
-
-
1
-
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
3
-
-
-
-
10
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
10
10
694711
Loscos
Ascorbate and homoglutathione ...
Phaseolus vulgaris
Plant Physiol.
146
1282-1292
2008
-
-
-
-
-
-
1
-
-
1
-
2
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676633
Naya
The response of carbon metabol ...
Medicago sativa
Plant Physiol.
144
1104-1114
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676726
Innocenti
Glutathione synthesis is regul ...
Medicago truncatula
Planta
225
1597-1602
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662922
Frendo
Glutathione and homoglutathion ...
Medicago truncatula
Mol. Plant Microbe Interact.
18
254-259
2005
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663073
Sugiyama
Homoglutathione confers tolera ...
Glycine max
Plant Cell Physiol.
46
1428-1432
2005
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648902
Iturbe-Ormaetxe
Cloning and functional charact ...
Pisum sativum
Physiol. Plant.
115
69-73
2002
-
-
-
-
-
-
-
2
1
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648904
Frendo
A Medicago truncatula homoglut ...
Medicago truncatula
Plant Physiol.
126
1706-1715
2001
-
-
1
-
2
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648906
Moran
Glutathione and homoglutathion ...
Phaseolus vulgaris, Pisum sativum
Plant Physiol.
124
1381-1392
2000
-
-
2
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648903
Matamoros
Glutathione and homoglutathion ...
Glycine max, Medicago sativa, no activity in Lupinus luteus, no activity in Vicia faba, no activity in Vigna radiata, Phaseolus vulgaris, Vigna radiata
Plant Physiol.
121
879-888
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648905
Frendo
-
Localization of glutathione an ...
Medicago truncatula
Plant J.
17
215-219
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648901
Klapheck
-
Properties and localization of ...
Phaseolus coccineus
Physiol. Plant.
74
733-739
1988
-
-
-
-
-
-
-
3
1
2
-
1
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
1
2
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1185
Macnicol
-
Homoglutathione and glutathion ...
Vigna radiata var. radiata
Plant Sci.
53
229-235
1987
-
-
-
-
-
-
6
5
-
1
1
1
-
1
-
-
1
-
-
1
1
1
3
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
5
-
1
1
1
-
-
-
1
-
1
1
1
3
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-