Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-phosphosulfate | 5 mM: 10-30% inhibition | Vigna radiata var. radiata | |
ADP | 5 mM: 70% inhibition | Vigna radiata var. radiata | |
AMP | 5 mM: 10-30% inhibition | Vigna radiata var. radiata | |
cysteine | 5 mM: 15% inhibition | Vigna radiata var. radiata | |
glutathione | 5 mM: 15% inhibition | Vigna radiata var. radiata | |
additional information | methionine, homocysteine, S-adenosyl-methionine, serine, all 5 mM, are no inhibitors | Vigna radiata var. radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
beta-Alanine | - |
Vigna radiata var. radiata | |
0.45 | - |
ATP | - |
Vigna radiata var. radiata | |
0.75 | - |
gamma-glutamylcysteine | - |
Vigna radiata var. radiata | |
7.5 | - |
glycine | - |
Vigna radiata var. radiata | |
8.3 | - |
gamma-aminobutyrate | - |
Vigna radiata var. radiata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Vigna radiata var. radiata | |
Mg2+ | 2-3 mM optimal | Vigna radiata var. radiata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
gel filtration | Vigna radiata var. radiata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine | Vigna radiata var. radiata | - |
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine | i.e. homoglutathione | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata var. radiata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Vigna radiata var. radiata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aerial part | - |
Vigna radiata var. radiata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.042 | - |
gamma-glutamylcysteine + beta-alanine | Vigna radiata var. radiata |
Storage Stability | Organism |
---|---|
-20°C, more than 1 mg enzyme/ml in potassium phosphate buffer, 30-50% glycerol, 1 mM dithiothreitol, stable for at least 4 months | Vigna radiata var. radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-glutamylcysteine + gamma-aminobutyrate | - |
Vigna radiata var. radiata | ADP + phosphate + gamma-glutamylcysteinyl-4-aminobutyrate | - |
? | |
ATP + gamma-glutamylcysteine + glycine | - |
Vigna radiata var. radiata | ADP + phosphate + gamma-glutamylcysteinylglycine | - |
? | |
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine | - |
Vigna radiata var. radiata | ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine | i.e. homoglutathione | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Vigna radiata var. radiata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
20 h, 50 mM potassium phosphate buffer, pH 7.5: 15% remaining activity. 20 h, buffer plus 10% (v/v) glycerol: 30% remaining activity. 20 h, buffer with glycerol plus 1 mM dithiothreitol: 50% remaining activity. 20 h, buffer with glycerol and dithiothreitol plus 10 mg/ml bovine serum albumin: 85% remaining activity | Vigna radiata var. radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Vigna radiata var. radiata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | 50% of maximal activity at pH 7 and pH 10 | Vigna radiata var. radiata |