Literature summary for 6.3.2.2 extracted from

Orellana, C.A.; Marcellin, E.; Gray, P.P.; Nielsen, L.K.
Overexpression of the regulatory subunit of glutamate-cysteine ligase enhances monoclonal antibody production in CHO cells (2017), Biotechnol. Bioeng., 114, 1825-1836 .

Cloned(Commentary)

Cloned (Comment)	Organism
genes I79_010621 or Gclc and I79_022778 or Gclm, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR expression analysis of recombinant and wild-type enzyme subunits, determination of isozyme Gclc-X2 lacking the first 5 amino acids compared to isozymes Gclc-X0 and Gclc-X1, recombinant overexpression of the enzyme subunits in a CHO-T cell line Epi-CHO, derived from CHO-K1 cells. Gclc-X2 but not Gclm overexpression increases Gcl activity	Cricetulus griseus

Cloned (Comment)

Organism

genes I79_010621 or Gclc and I79_022778 or Gclm, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR expression analysis of recombinant and wild-type enzyme subunits, determination of isozyme Gclc-X2 lacking the first 5 amino acids compared to isozymes Gclc-X0 and Gclc-X1, recombinant overexpression of the enzyme subunits in a CHO-T cell line Epi-CHO, derived from CHO-K1 cells. Gclc-X2 but not Gclm overexpression increases Gcl activity

Cricetulus griseus

Protein Variants

Protein Variants	Comment	Organism
additional information	overexpression of subunit Gclc-X2 leads to upregulation of the proteins Abcf1, Fkbp4, and Eif3h, as well as to downregulation of protein Lamb1. There is no significant difference in growth rate during exponential phase at 32C between Gclm+ or Gclc-X2+ populations and the wild-type population. A higher cell proliferation observed in the Gclc overexpressing population	Cricetulus griseus

Organism

Organism	UniProt	Comment	Textmining
Cricetulus griseus	G3HIY9 AND G3IG96	genes I79_010621 and I79_022778 encoding catalytic and regulatory subunits	-

Synonyms

Synonyms	Comment	Organism
GCL	-	Cricetulus griseus
GCLC	-	Cricetulus griseus
Gclc-X2	-	Cricetulus griseus
GCLM	-	Cricetulus griseus
I79_022778	-	Cricetulus griseus

General Information

General Information	Comment	Organism
malfunction	overexpression of subunit Gclc-X2 leads to upregulation of the proteins Abcf1, Fkbp4, and Eif3h, as well as to downregulation of protein Lamb1. There is no significant difference in growth rate during exponential phase at 32C between Gclm+ or Gclc-X2+ populations and the wild-type population. A higher cell proliferation observed in the Gclc overexpressing population. Gclc-X2 but not Gclm overexpression increases Gcl activity	Cricetulus griseus
metabolism	the enzyme catalyzes the first step in the glutathione biosynthesis	Cricetulus griseus