Cloned (Comment) | Organism |
---|---|
gene gshF, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Actinobacillus pleuropneumoniae |
gene gshF, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Actinobacillus succinogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GSH | feedback inhibition, the gamma-GCS activity is inhibited by a high concentration of GSH | Actinobacillus pleuropneumoniae | |
GSH | feedback inhibition, the gamma-GCS activity is inhibited by a high concentration of GSH | Actinobacillus succinogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Actinobacillus pleuropneumoniae | |
additional information | - |
additional information | kinetics | Actinobacillus succinogenes | |
0.05 | - |
L-cysteine | pH 8.5, 40°C, recombinant His-tagged enzyme | Actinobacillus succinogenes | |
0.199 | - |
ATP | pH 8.5, 40°C, recombinant His-tagged enzyme | Actinobacillus pleuropneumoniae | |
0.385 | - |
ATP | pH 8.5, 40°C, recombinant His-tagged enzyme | Actinobacillus succinogenes | |
11 | - |
L-glutamate | pH 8.5, 40°C, recombinant His-tagged enzyme | Actinobacillus succinogenes | |
12 | - |
L-glutamate | pH 8.5, 40°C, recombinant His-tagged enzyme | Actinobacillus pleuropneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Actinobacillus pleuropneumoniae | |
Mg2+ | required | Actinobacillus succinogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + L-cysteine | Actinobacillus pleuropneumoniae | - |
ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | Actinobacillus succinogenes | - |
ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | Actinobacillus succinogenes ATCC 55618 | - |
ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | Actinobacillus pleuropneumoniae ATCC 55618 | - |
ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinobacillus pleuropneumoniae | D4N892 | - |
- |
Actinobacillus succinogenes | A6VQP8 | - |
- |
Actinobacillus succinogenes ATCC 55618 | A6VQP8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Actinobacillus pleuropneumoniae |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Actinobacillus succinogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
GshFAp has a gamma-GCS catalytic activity with Vmax of 820 U/mg | Actinobacillus pleuropneumoniae |
additional information | - |
GshFAs has a high gamma-GCS catalytic activity with Vmax of 1560 U/mg | Actinobacillus succinogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + L-cysteine | - |
Actinobacillus pleuropneumoniae | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | - |
Actinobacillus succinogenes | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | - |
Actinobacillus succinogenes ATCC 55618 | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? | |
ATP + L-glutamate + L-cysteine | - |
Actinobacillus pleuropneumoniae ATCC 55618 | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Asuc_1947 | - |
Actinobacillus pleuropneumoniae |
Asuc_1947 | - |
Actinobacillus succinogenes |
bifunctional L-glutathione synthetase | - |
Actinobacillus pleuropneumoniae |
bifunctional L-glutathione synthetase | - |
Actinobacillus succinogenes |
gamma-GCS | - |
Actinobacillus pleuropneumoniae |
glutathione biosynthesis bifunctional protein GshAB | UniProt | Actinobacillus pleuropneumoniae |
glutathione biosynthesis bifunctional protein GshAB | UniProt | Actinobacillus succinogenes |
gshAB | gene name, UniProt | Actinobacillus pleuropneumoniae |
gshAB | gene name, UniProt | Actinobacillus succinogenes |
GshF | - |
Actinobacillus pleuropneumoniae |
GshF | - |
Actinobacillus succinogenes |
GshFAp | - |
Actinobacillus pleuropneumoniae |
GshFAs | - |
Actinobacillus succinogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Actinobacillus pleuropneumoniae |
40 | - |
- |
Actinobacillus succinogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Actinobacillus pleuropneumoniae |
9 | - |
- |
Actinobacillus succinogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Actinobacillus pleuropneumoniae | |
ATP | - |
Actinobacillus succinogenes |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
GSH | pH 8.5, 40°C | Actinobacillus pleuropneumoniae | |
0.043 | - |
GSH | pH 8.5, 40°C, recombinant His-tagged enzyme | Actinobacillus succinogenes |