Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.2 extracted from

  • Backos, D.S.; Fritz, K.S.; Roede, J.R.; Petersen, D.R.; Franklin, C.C.
    Posttranslational modification and regulation of glutamate-cysteine ligase by the alpha,beta-unsaturated aldehyde 4-hydroxy-2-nonenal (2011), Free Radic. Biol. Med., 50, 14-26.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.2

Activating Compound

Activating Compound Comment Organism Structure
4-hydroxy-2-nonenal 4-hydroxy-2-nonenal causes a rapid dose- and time-dependent increase in A549 cell GCL activity. Maximal activation of GCL occurs at 30 min in response to 50 microM 4-hydroxy-2-nonenal Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
both regulatory subunit GCLM and catalytic subunit GCLC Mus musculus
both regulatory subunit GCLM and catalytic subunit GCLC Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxy-2-nonenal treatment with 4-hydroxy-2-nonenal results in the dose-dependent adduction of both monomeric GCLC and GCLM. 4-Hydroxy-2-nonenal-mediated adduction of monomeric GCLC results in a dose-dependent increase in GCLC enzymatic activity. Treatment of GCL holoenzyme causes a dose-dependent decrease in GCL activity. 4-Hydroxy-2-nonenal-mediated inhibition of GCL holoenzyme activity is associated with a reduction in the levels of heterodimeric GCL holoenzyme complex due to increase in high molecular weight complexes. 4-Hydroxy-2-nonenal modification simultaneously activates monomeric GCLC activity and prevents its ability to heterodimerize with GCLM and form functional GCL holoenzyme Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information 4-hydroxy-2-nonenal alters GCL holoenzyme formation and activity via direct posttranslational modification of the GCL subunits in vitro. 4-Hydroxy-2-nonenal directly modifies Cys553 of catalytic subunit GCLC and Cys35 of modulatory subunit GCLM in vitro, which significantly increases monomeric GCLC enzymatic activity, but reduces GCL holoenzyme activity and formation of the GCL holoenzymecomplex Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
GCL
-
 Mus musculus
GCLC
-
 Mus musculus
GCLM
-
 Mus musculus