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Literature summary for 6.3.2.2 extracted from

  • Sharma, S.K.; Banyal, H.S.
    gamma-glutamylcysteine synthetase from Plasmodium berghei (2009), Parasitol. Int., 58, 145-153.
    View publication on PubMed
Search for more literature for 6.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inactivation Plasmodium berghei
Cd2+ no residual activity Plasmodium berghei
Co2+ 22% residual activity Plasmodium berghei
Cu2+ 27% residual activity Plasmodium berghei
glutathione competitive to L-Glu, non-competitive to ATP and L-Cys Plasmodium berghei
Hg2+ no residual activity Plasmodium berghei
Ni2+ complete inactivation Plasmodium berghei
Zn2+ 21% residual activity Plasmodium berghei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
 L-Cys pH 8.2 Plasmodium berghei
0.75
-
 L-Glu pH 8.2 Plasmodium berghei
0.92
-
 ATP pH 8.2 Plasmodium berghei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Plasmodium berghei 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ absolute requirement for Mg2+, Mn2+, and K+ ions Plasmodium berghei
Mg2+ absolute requirement for Mg2+, Mn2+, and K+ ions Plasmodium berghei
Mn2+ absolute requirement for Mg2+, Mn2+, and K+ ions Plasmodium berghei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 1 * 66000 + 1 * 57000, SDS-PAGE Plasmodium berghei
66000
-
 1 * 66000 + 1 * 57000, SDS-PAGE Plasmodium berghei
120000
-
 PAGE Plasmodium berghei

Organism

Organism UniProt Comment Textmining
Plasmodium berghei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Plasmodium berghei

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39.5
-
 pH 8.2 Plasmodium berghei

Storage Stability

Storage Stability Organism
4°C, stable for up to 96 h Plasmodium berghei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + L-Cys
-
 Plasmodium berghei ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 66000 + 1 * 57000, SDS-PAGE Plasmodium berghei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.53
-
 glutathione pH 8.2 Plasmodium berghei