Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | In response to redox environment, AtGCL undergoes a reversible conformational change that modulates the enzymatic activity of the monomer | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-85 | mutant lacking the N-terminal localization sequence | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
buthionine sulfoximine | - |
Arabidopsis thaliana | |
cystamine | - |
Arabidopsis thaliana | |
glutathione | feedback inhibition, non-competitive inhibitor versus both glutamate and cysteine | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
cysteine | pH 7.0, 25°C, mutant DELTA85 | Arabidopsis thaliana | |
9.1 | - |
glutamate | pH 7.0, 25°C, mutant DELTA85 | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | 65000 | recombinant full-length enzyme, gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P46309 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glutamate + L-cysteine | random ter-reactant mechanism with a preferred binding order | Arabidopsis thaliana | ADP + phosphate + gamma-L-glutamyl-L-cysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtGCL | - |
Arabidopsis thaliana |
GCL | - |
Arabidopsis thaliana |
glutamate-cysteine ligase | - |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.72 | - |
glutathione | pH 7.0, 25°C, versus glutamate, mutant DELTA85 | Arabidopsis thaliana | |
1.2 | - |
buthionine sulfoximine | pH 7.0, 25°C, mutant DELTA85 | Arabidopsis thaliana | |
2.21 | - |
glutathione | pH 7.0, 25°C, versus cysteine, mutant DELTA85 | Arabidopsis thaliana | |
11 | - |
cystamine | pH 7.0, 25°C, mutant DELTA85 | Arabidopsis thaliana |