Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.2 extracted from

  • Jez, J.M.; Cahoon, R.E.; Chen, S.
    Arabidopsis thaliana glutamate-cysteine ligase: functional properties, kinetic mechanism, and regulation of activity (2004), J. Biol. Chem., 279, 33463-33470.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information In response to redox environment, AtGCL undergoes a reversible conformational change that modulates the enzymatic activity of the monomer Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
DELTA1-85 mutant lacking the N-terminal localization sequence Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
buthionine sulfoximine
-
Arabidopsis thaliana
cystamine
-
Arabidopsis thaliana
glutathione feedback inhibition, non-competitive inhibitor versus both glutamate and cysteine Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
cysteine pH 7.0, 25°C, mutant DELTA85 Arabidopsis thaliana
9.1
-
glutamate pH 7.0, 25°C, mutant DELTA85 Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000 65000 recombinant full-length enzyme, gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P46309
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glutamate + L-cysteine random ter-reactant mechanism with a preferred binding order Arabidopsis thaliana ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?

Subunits

Subunits Comment Organism
monomer
-
Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtGCL
-
Arabidopsis thaliana
GCL
-
Arabidopsis thaliana
glutamate-cysteine ligase
-
Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.72
-
glutathione pH 7.0, 25°C, versus glutamate, mutant DELTA85 Arabidopsis thaliana
1.2
-
buthionine sulfoximine pH 7.0, 25°C, mutant DELTA85 Arabidopsis thaliana
2.21
-
glutathione pH 7.0, 25°C, versus cysteine, mutant DELTA85 Arabidopsis thaliana
11
-
cystamine pH 7.0, 25°C, mutant DELTA85 Arabidopsis thaliana