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Literature summary for 6.3.2.2 extracted from
- Enhanced expression of pulmonary gamma-glutamylcysteine synthetase heavy subunit in rats exposed to cadmium aerosols (2000), Toxicol. Appl. Pharmacol., 163, 249-259.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cadmium aerosols | 2.4 mg Cd/m3, enhance in the lung the expression of the enzyme's heavy, catalytic subunit gamma-GCS-HS by 4.5fold after 15 min, 8fold after 6 h, increase in enzyme activity and GSH production rate | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-buthionine-SR-sulfoximine | specific | Rattus norvegicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
x * 73000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + L-Cys | Rattus norvegicus | first and rate limiting step in GSH de novo biosynthesis | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Lewis rats | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| alveolus | corners of, highest enzyme content | Rattus norvegicus | - |
| epithelium | alveolar, higher content compared to the lung parenchyma | Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| additional information | pulmonary distribution | Rattus norvegicus | - |
| parenchyma | lower content compared to the alveolar epithelium | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0027 | - |
lung extract exposed to air | Rattus norvegicus |
| 0.0041 | - |
lung extract exposed to cadmium aerosol with 3.3 mg Cd/m3 | Rattus norvegicus |
| 0.0048 | - |
lung extract exposed to cadmium aerosol with 4.2 mg Cd/m3 | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + L-Cys | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
| ATP + L-Glu + L-Cys | first and rate limiting step in GSH de novo biosynthesis | Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 73000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-GCS | - |
Rattus norvegicus |
| gamma-Glutamylcysteine synthetase | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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