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Literature summary for 6.3.2.2 extracted from

  • Huang, C.S.; Chang, L.S.; Anderson, M.E.; Meister, A.
    Catalytic and regulatory properties of the heavy subunit of rat kidney gamma-glutamylcysteine synthetase (1993), J. Biol. Chem., 268, 19675-19680.
    View publication on PubMed
Search for more literature for 6.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
cDNA for heavy subunit expressed in Escherichia coli Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
gamma-Glu-2-aminobutanoyl-Gly i.e. ophthalmic acid, inhibits only slightly, but inhibits much more after treatment of the holoenzyme with DTT, the recombinant and isolated heavy subunit enzyme is substantially inhibited without DTT Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 L-Cys L-Cys Rattus norvegicus
0.8
-
 L-2-aminobutanoate recombinant heavy subunit Rattus norvegicus
1.2
-
 L-Glu L-2-aminobutanoate Rattus norvegicus
1.4
-
 L-2-aminobutanoate L-Glu, holoenzyme Rattus norvegicus
18.2
-
 L-Glu recombinant heavy subunit Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27700
-
 1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT Rattus norvegicus
73000
-
 1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + L-Cys
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT Rattus norvegicus
More the light subunit has a regulatory function affecting the affinity for Glu and GSH Rattus norvegicus