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Literature summary for 6.3.2.17 extracted from

  • Meng, H.; Jiang, L.; Xu, B.; Guo, W.; Li, J.; Zhu, X.; Qi, X.; Duan, L.; Meng, X.; Fan, Y.; Zhang, C.
    Arabidopsis plastidial folylpolyglutamate synthetase is required for seed reserve accumulation and seedling establishment in darkness (2014), PLoS ONE, 9, e101905 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.17

Protein Variants

Protein Variants Comment Organism
additional information generation of a T-DNA insertion mutant (atdfb-3) of the plastidial folylpolyglutamate synthetase gene (AtDFB). The mutant is defective in seed reserves and skotomorphogenesis. Lower carbon and higher nitrogen content in the mutant seeds compared to wild-type are indicative of an altered C and N partitioning capacity. Higher levels of organic acids and sugars are detected in the mutant seeds compared with the wild-type. Mutant atdfb-3 seeds contain less total amino acids and individual Asn and Glu as well as NO3-. The mutant exhibts significant changes in seed storage. Defects in hypocotyl elongation are observed in atdfb-3 in darkness under sufficient nitrate conditions, and further enhances under nitrate limited conditions. Mutant sensitivity to limited nitrate during a narrow developmental window. Exogenous 5-formyl-tetrahydrofolate completely restores the hypocotyl length in atdfb-3 seedlings with nitrate as the sole N source. Further study demonstrated that folate profiling and N metabolism are perturbed in atdfb-3 etiolated seedlings. The activity of enzymes involved in N reduction and assimilation is altered in atdfb-3. Exogenous 5-fluoro-tetrahydrofolate restores the wild-type hypocotyl phenotype in etiolated atdfb-3 mutant seedlings Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate Arabidopsis thaliana
-
 ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana F4K2A1
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Arabidopsis thaliana
-
hypocotyl
-
 Arabidopsis thaliana
-
additional information strong expression of the enzyme in cotyledons and hypocotyl during early developmental stage, histochemical localization of AtDFB promoter activity and AtDFB expression patterns, overview Arabidopsis thaliana
-
seed
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
-
 Arabidopsis thaliana ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
 ?

Synonyms

Synonyms Comment Organism
AtDFB
-
 Arabidopsis thaliana
Folylpolyglutamate synthetase
-
 Arabidopsis thaliana
FPGS1
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction a T-DNA insertion mutant (atdfb-3 or fpgs1) of the plastidial folylpolyglutamate synthetase gene (AtDFB) is defective in seed reserves and skotomorphogenesis. Lower carbon and higher nitrogen content in the mutant seeds compared to wild-type are indicative of an altered C and N partitioning capacity. Higher levels of organic acids and sugars are detected in the mutant seeds compared with the wild-type. Mutant atdfb-3 seeds contain less total amino acids and individual Asn and Glu as well as NO3-. The mutant exhibts significant changes in seed storage. Defects in hypocotyl elongation are observed in atdfb-3 in darkness under sufficient nitrate conditions, and further enhances under nitrate limited conditions. Mutant sensitivity to limited nitrate during a narrow developmental window. Exogenous 5-formyl-tetrahydrofolate completely restores the hypocotyl length in atdfb-3 seedlings with nitrate as the sole N source. Further study demonstrated that folate profiling and N metabolism are perturbed in atdfb-3 etiolated seedlings. The activity of enzymes involved in N reduction and assimilation is altered in atdfb-3. Metabolite profiles in wild-type and atdfb-3 seeds, phenotype, overview Arabidopsis thaliana
physiological function the enzyme is required for seed reserves, hypocotyl elongation, and N metabolism in darkness, providing novel insights into potential associations of folate metabolism with seed reserve accumulation, N metabolism, and hypocotyl development in Arabidopsis thaliana Arabidopsis thaliana