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Literature summary for 6.3.2.17 extracted from

  • Meng, H.; Xu, B.; Zhang, C.; Jiang, L.
    Arabidopsis plastidial folylpolyglutamate synthetase is required for nitrogen metabolism under nitrate-limited condition in darkness (2017), Biochem. Biophys. Res. Commun., 482, 277-281 .
    View publication on PubMed
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Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana F4K2A1
-
-
Arabidopsis thaliana Col-0 F4K2A1
-
-

Synonyms

Synonyms Comment Organism
AtDFB
-
 Arabidopsis thaliana
folylpolyglutamate synthetase 1
-
 Arabidopsis thaliana
FPGS
-
 Arabidopsis thaliana
FPGS1
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction a T-DNA insertion mutant (atdfb-3) of the plastidial folylpolyglutamate synthetase gene (AtDFB) is defective in folate metabolism and nitrogen metabolism under nitrate-limited conditions in darkness. Exogenous applied 5-formyl-tetrahydrofolate (5-F-THF) completely restores nitrogen content, soluble protein, total amino acids, individual amino acids including Glu, Gln, Asp, Asn, Pro, Arg and Met, nitrate, and endogenous 5-F-THF in atdfb-3 to the wild-type level. Ser in atdfb-3 is 19.94fold higher than that in the wild-type, the content of 5-F-THF in the wild-type and atdfb-3is 203.40fold and 120.75fold, respectively, of that without 5-F-THF application. At the same time the application of 5-F-THF partially restored the content of Ser and nitrite in the mutant. The mutant phenotype shows atdfb-dramatically shortened hypocotyls, altered nitrogen and folate metabolism under nitrate-limited condition, and exogenous applied 5-F-THF completely restored hypocotyl length in atdfb-3 seedlings with nitrate as the sole nitrogen source Arabidopsis thaliana
physiological function intact folate metabolism is necessary for nitrogen metabolism in Arabidopsis thaliana under nitrate-limited condition in darkness, providing novel insights into function of folate Arabidopsis thaliana