Cloned (Comment) | Organism |
---|---|
expressed in AuxB1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A447V | does not affect enzyme activity | Homo sapiens |
R424C | reduced efficacy with substrates compared to the wild type enzyme | Homo sapiens |
S457F | reduced efficacy with substrates compared to the wild type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | - |
Homo sapiens | |
pemetrexed | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0526 | - |
methotrexate | wild type enzyme from mitochondrion | Homo sapiens | |
0.0555 | - |
methotrexate | wild type enzyme from cytosol | Homo sapiens | |
0.0613 | - |
methotrexate | mutant enzyme S457F | Homo sapiens | |
0.0827 | - |
methotrexate | mutant enzyme A447V | Homo sapiens | |
0.0908 | - |
methotrexate | mutant enzyme R424C | Homo sapiens | |
1.63 | - |
Glu | mutant enzyme A447V | Homo sapiens | |
1.702 | - |
Glu | wild type enzyme from cytosol | Homo sapiens | |
2.068 | - |
Glu | wild type enzyme from mitochondrion | Homo sapiens | |
2.281 | - |
Glu | mutant enzyme S457F | Homo sapiens | |
5.984 | - |
Glu | mutant enzyme R424C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + methotrexate + Glu | - |
Homo sapiens | ADP + phosphate + methotrexyl-Glu | - |
? | |
ATP + pemetrexed + Glu | - |
Homo sapiens | ADP + phosphate + pemetrexyl-Glu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Folylpolyglutamate synthase | - |
Homo sapiens |
FPGS | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000095 | - |
wild type enzyme from cytosol | Homo sapiens | methotrexate | |
0.000011 | - |
wild type enzyme from mitochondrion | Homo sapiens | pemetrexed | |
0.0000127 | - |
mutant enzyme A447V | Homo sapiens | pemetrexed | |
0.0000129 | - |
mutant enzyme A447V | Homo sapiens | methotrexate | |
0.0000136 | - |
wild type enzyme from cytosol | Homo sapiens | pemetrexed | |
0.0000145 | - |
wild type enzyme from mitochondrion | Homo sapiens | methotrexate | |
0.0000156 | - |
mutant enzyme S457F | Homo sapiens | methotrexate | |
0.0000175 | - |
mutant enzyme R424C | Homo sapiens | methotrexate | |
0.0000263 | - |
mutant enzyme R424C | Homo sapiens | pemetrexed | |
0.0000302 | - |
mutant enzyme S457F | Homo sapiens | pemetrexed |