Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lacticaseibacillus casei |
Protein Variants | Comment | Organism |
---|---|---|
D313A | mutation in the C-terminal of the enzyme | Lacticaseibacillus casei |
E143A | mutation in the MgATP2- binding site of the enzyme | Lacticaseibacillus casei |
E143D | mutation in the MgATP2- binding site of the enzyme | Lacticaseibacillus casei |
E143Q | mutation in the MgATP2- binding site of the enzyme | Lacticaseibacillus casei |
E81A | mutation in the omega-loop of the enzyme | Lacticaseibacillus casei |
G411A | mutation in the C-terminal of the enzyme | Lacticaseibacillus casei |
H316A | mutation in the C-terminal of the enzyme | Lacticaseibacillus casei |
K185A | mutation in the MgATP2- binding site of the enzyme | Lacticaseibacillus casei |
P74A | mutation in the omega-loop of the enzyme | Lacticaseibacillus casei |
R82A | mutation in the omega-loop of the enzyme | Lacticaseibacillus casei |
S412A | mutation in the C-terminal of the enzyme | Lacticaseibacillus casei |
S73A | mutation in the omega-loop of the enzyme | Lacticaseibacillus casei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0315 | - |
5,6,7,8-tetrahydrofolic acid | pH 7.5, wild type enzyme | Lacticaseibacillus casei | |
0.071 | - |
5,6,7,8-tetrahydrofolic acid | pH 7.5, E81A mutant | Lacticaseibacillus casei | |
0.094 | - |
5,6,7,8-tetrahydrofolic acid | pH 7.5, S412A mutant | Lacticaseibacillus casei | |
0.21 | - |
5,6,7,8-tetrahydrofolic acid | pH 7.5, R82A mutant | Lacticaseibacillus casei | |
0.24 | - |
5,6,7,8-tetrahydrofolic acid | pH 7.5, K185A mutant | Lacticaseibacillus casei | |
0.41 | - |
L-glutamate | pH 7.5, E81A mutant | Lacticaseibacillus casei | |
0.47 | - |
L-glutamate | pH 7.5, wild type enzyme | Lacticaseibacillus casei | |
0.62 | - |
ATP | pH 7.5, S412A mutant | Lacticaseibacillus casei | |
0.655 | - |
5,6,7,8-tetrahydrofolic acid | pH 7.5, S73A mutant | Lacticaseibacillus casei | |
0.85 | - |
L-glutamate | pH 7.5, S73A mutant | Lacticaseibacillus casei | |
0.87 | - |
ATP | pH 7.5, R82A mutant | Lacticaseibacillus casei | |
2.06 | - |
L-glutamate | pH 7.5, R82A mutant | Lacticaseibacillus casei | |
2.5 | - |
ATP | pH 7.5, S73A mutant | Lacticaseibacillus casei | |
2.9 | - |
ATP | pH 7.5, K185A mutant | Lacticaseibacillus casei | |
3.4 | - |
ATP | pH 7.5, wild type enzyme | Lacticaseibacillus casei | |
5.06 | - |
L-glutamate | pH 7.5, K185A mutant | Lacticaseibacillus casei | |
5.2 | - |
ATP | pH 7.5, E81A mutant | Lacticaseibacillus casei | |
26.69 | - |
L-glutamate | pH 7.5, S412A mutant | Lacticaseibacillus casei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | P15925 | - |
- |
Purification (Comment) | Organism |
---|---|
chromatography on a chitin column | Lacticaseibacillus casei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0015 | - |
K185A mutant | Lacticaseibacillus casei |
0.0043 | - |
S412A mutant | Lacticaseibacillus casei |
0.0053 | - |
E81A mutant | Lacticaseibacillus casei |
0.008 | - |
R82A mutant | Lacticaseibacillus casei |
0.0093 | - |
S73A mutant | Lacticaseibacillus casei |
0.2 | - |
wild type enzyme | Lacticaseibacillus casei |
Storage Stability | Organism |
---|---|
-70ºC, 20% DMSO, stable | Lacticaseibacillus casei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate | - |
Lacticaseibacillus casei | ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu | - |
? |