Literature summary for 6.3.2.17 extracted from

  • Moran, R.G.; Colman, P.D.
    Mammalian folyl polyglutamate synthetase: Partial purification and properties of the mouse liver enzyme (1984), Biochemistry, 23, 4580-4589.
    View publication on PubMed

General Stability

General Stability Organism
stabilized by glycerol or by ATP Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Mus musculus
AMP
-
Mus musculus
diphosphate
-
Mus musculus
(NH4)2SO4
-
Mus musculus
phosphate
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.137
-
pteroyl-Glu
-
Mus musculus
0.007
-
5,6,7,8-tetrahydropteroyl-Glu
-
Mus musculus
0.0081
-
5-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
Mus musculus
0.0039
-
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
Mus musculus
0.0048
-
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
-
Mus musculus
0.0086
-
7,8-dihydropteroyl-Glu
-
Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
liver, partial Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information most effective substrates are: 7,8-dihydrofolate, 5,6,7,8-tetrahydrofolate, 5,10-methylenetetrahydrofolate, 10-formyltetrahydrofolate, 5-formyltetrahydrofolate Mus musculus ?
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Mus musculus ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n:1 Mus musculus ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
Mus musculus ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
Mus musculus ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu n: 1 Mus musculus ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
?
ATP + pteroyl-Glu + Glu
-
Mus musculus ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
Mus musculus ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
-
Mus musculus ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
?
ADP + phosphate + 7,8-dihydropteroyl-Glu
-
Mus musculus ATP + 7,8-dihydropteroate + L-glutamate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 9.5
-
Mus musculus