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Literature summary for 6.3.2.17 extracted from
- Mammalian folylpoly-gamma-glutamate synthetase. 1. Purification and general properties of the hog liver enzyme (1987), Biochemistry, 26, 504-512.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | maximal stimulation at 25 mM | Sus scrofa |  |
| 2-mercaptoethanol | inhbibition above 100 mM | Sus scrofa | |
| dithiothreitol | maximal stimulation at 2.5 mM | Sus scrofa | |
| Reducing agents | absolutely dependent on | Sus scrofa | |
| Reducing agents | beta-mercaptoethanol or DTT | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| K+ | required at low concentration, inhibition at high concentration | Sus scrofa | |
| NH4+ | required at low concentration, inhibition at high concentration | Sus scrofa | |
| Rb+ | required at low concentration, inhibition at high concentration | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
ATP | ATP | Sus scrofa | |
| 0.332 | - |
Glu | - |
Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | absolutely dependent on monovalent cation, K+ is most effective | Sus scrofa | |
| K+ | inhibition at high concentrations | Sus scrofa | |
| Mg2+ | required | Sus scrofa | |
| Mg2+ | in presence of 0.1 mM ATP, maximal activity at 2 mM Mg2+ | Sus scrofa | |
| Mn2+ | supports activity, at 1 mM 68% of the activity obtained with Mg2+ | Sus scrofa | |
| NH4+ | stimulates to a lesser extent than K+ | Sus scrofa | |
| NH4+ | inhibition by high concentrations | Sus scrofa | |
| Rb+ | absolute requirement for monovalent cation is met by, inhibition at high concentrations | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
hog | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| two forms | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.465 | - |
- |
Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu | n: 1 | Sus scrofa | ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1 | - |
? | |
| ATP + 7,8-dihydropteroate + Glu | - |
Sus scrofa | ADP + phosphate + 7,8-dihydropteroyl-Glu | - |
? | |
| ATP + 7,8-dihydropteroyl-Glun + Glu | n: 1 | Sus scrofa | ADP + phosphate + 7,8-dihydropteroyl-Glun+1 | - |
? | |
| ATP + aminopterin + Glu | - |
Sus scrofa | ADP + phosphate + aminopteryl-Glu | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.4 | - |
- |
Sus scrofa |
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