Literature summary for 6.3.2.17 extracted from

  • Cichowicz, D.J.; Shane, B.
    Mammalian folylpoly-gamma-glutamate synthetase. 2. Substrate specificity and kinetic properties (1987), Biochemistry, 26, 513-521.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-threo-Fluoroglutamate prevents or severly retards further addition of Glu Sus scrofa
adenosine 5'-O-(3-thiotriphosphate)
-
Sus scrofa
ADP
-
Sus scrofa
aminopterin
-
Sus scrofa
AMP
-
Sus scrofa
ATP4-
-
Sus scrofa
beta,gamma-Imido-ATP
-
Sus scrofa
beta,gamma-methylene-ATP
-
Sus scrofa
diphosphate weak, competitive with respect to MgATP2- Sus scrofa
L-Homocysteate prevents or severly retards further addition of Glu Sus scrofa
P1,P5-di(adenosine-5')pentaphosphate
-
Sus scrofa
phosphate competitive with respect to MgATP2- Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
dATP reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP Sus scrofa
0.057
-
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
-
Sus scrofa
0.062
-
pteroylmonoglutamyl-Glu pteroyl-Glu2 Sus scrofa
0.0011
-
5,6,7,8-tetrahydropteroyl-Glu3 5,6,7,8-tetrahydropteroyl-Glu5 Sus scrofa
0.002
-
5,6,7,8-tetrahydropteroyl-Glu4
-
Sus scrofa
0.005
-
7,8-dihydropteroyl-Glu
-
Sus scrofa
0.0026
-
7,8-dihydropteroyl-Glu2
-
Sus scrofa
0.19
-
UTP reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP Sus scrofa
0.01
-
ATP reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP Sus scrofa
0.017
-
aminopterin
-
Sus scrofa
0.006
-
5,6,7,8-tetrahydropteroyl-Glu
-
Sus scrofa
0.336
-
5,6,7,8-tetrahydropteroyl-Glu
-
Sus scrofa
0.0022
-
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
Sus scrofa
0.0034
-
5,6,7,8-tetrahydropteroyl-Glu2
-
Sus scrofa
0.0027
-
5,6,7,8-tetrahydropteroyl-Glu5
-
Sus scrofa
0.0077
-
5,6,7,8-tetrahydropteroyl-Glu
-
Sus scrofa
0.054
-
methotrexate 5-methyl-5,6,7,8-tetrahydrpteroyl-Glu Sus scrofa
0.115
-
pteroyl-Glu
-
Sus scrofa
0.119
-
pteroyl-Glu3
-
Sus scrofa
0.136
-
ITP reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP Sus scrofa
0.346
-
Glu reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP Sus scrofa
0.962
-
pteroylglutamate
-
Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
hog
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + aminopterin + Glu
-
Sus scrofa ADP + phosphate + aminopteryl-Glu
-
?
ATP + methotrexate + Glu
-
Sus scrofa ADP + phosphate + methotrexyl-Glu
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 3 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 5 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 2 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 7 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
-
Sus scrofa dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
?
ITP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
-
Sus scrofa IDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
?
UTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
-
Sus scrofa UDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n:1 Sus scrofa ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
Sus scrofa ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
Sus scrofa ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
?
ATP + 7,8-dihydropteroate + Glu
-
Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glu
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu n: 5 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu n: 2 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
?
ATP + pteroyl-Glu + Glu n: 3 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
ATP + pteroyl-Glu + Glu n: 5 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
ATP + pteroyl-Glu + Glu n: 2 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
ATP + pteroyl-Glu + Glu n: 4 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
ATP + pteroyl-Glu + Glu n: 7 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
ATP + pteroyl-Glu + Glu n: 6 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
ATP + pteroyl-Glu + Glu n: 1, i.e. pteroylmonoglutamate, folic acid Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
Sus scrofa ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-Glu
-
Sus scrofa ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
-
Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu4
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu4 + L-glutamate
-
Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu5
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
-
Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu6
-
?
ATP + pteroyl-Glu3 + L-glutamate
-
Sus scrofa ADP + phosphate + pteroyl-Glu4
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
aminopterin
-
Sus scrofa
1.7
-
L-Glu
-
Sus scrofa