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Literature summary for 6.3.2.17 extracted from

  • Shane, B.; Cichowicz, D.J.
    Folylpoly-gamma-glutamate synthetases: properties and regulation (1983), Adv. Exp. Med. Biol., 163, 149-165.
    View publication on PubMed
Search for more literature for 6.3.2.17

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Corynebacterium sp.
ADP
-
 Lacticaseibacillus casei
AMP
-
 Corynebacterium sp.
beta,gamma-methylene-ATP
-
 Corynebacterium sp.
beta,gamma-methylene-ATP
-
 Lacticaseibacillus casei
L-Homocysteate
-
 Corynebacterium sp.
phosphate
-
 Corynebacterium sp.
phosphate
-
 Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 5,6,7,8-tetrahydropteroyl-Glu
-
 Corynebacterium sp.
0.018
-
 5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu
-
 Corynebacterium sp.
0.16
-
 5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu L-Glu Corynebacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ absolutely dependent on monovalent cation, K+ is most effective Corynebacterium sp.
K+ absolutely dependent on monovalent cation, K+ is most effective Lacticaseibacillus casei
K+ maximal activity at 200 mM Corynebacterium sp.
K+ maximal activity at 200 mM Lacticaseibacillus casei
Mg2+ required Corynebacterium sp.
Mg2+ required Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 1 * 53000, SDS-PAGE Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Bacteria
-
-
-
Corynebacterium sp.
-
-
-
Cricetulus griseus
-
-
-
Enterococcus faecalis
-
-
-
Lacticaseibacillus casei
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate = ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1 sequential mechanism Corynebacterium sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.57
-
-
 Corynebacterium sp.

Storage Stability

Storage Stability Organism
-20°C or -196°C, 50 mM potassium phosphate buffer, pH 7, 50 mM KCl, 30% DMSO Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Corynebacterium sp. ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 3 Corynebacterium sp. ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 3 Lacticaseibacillus casei ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 2 Corynebacterium sp. ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 2 Lacticaseibacillus casei ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n:1 Corynebacterium sp. ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu + L-Glu
-
 Corynebacterium sp. ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu2
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Corynebacterium sp. ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Lacticaseibacillus casei ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Corynebacterium sp. ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 7,8-dihydropteroate + Glu
-
 Corynebacterium sp. ADP + phosphate + 7,8-dihydropteroyl-Glu
-
 ?
ATP + aminopterin + Glu
-
 Corynebacterium sp. ADP + phosphate + aminopteryl-Glu
-
 ?
ATP + aminopterin + Glu
-
 Lacticaseibacillus casei ADP + phosphate + aminopteryl-Glu
-
 ?
ATP + L-homocysteate + Glu
-
 Corynebacterium sp. ADP + phosphate + (6RS)-5-methyl-H4-homofolyl-Glu
-
 ?
ATP + tetrahydroaminopterin + Glu
-
 Corynebacterium sp. ADP + phosphate + tetrahydroaminopteryl-Glu
-
 ?
CTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu at 13% of the activity relative to ATP Corynebacterium sp. CDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu as effective as ATP Corynebacterium sp. dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
 ?
additional information
-
 Lacticaseibacillus casei ?
-
 ?
additional information enzyme also posseses dihydrofolate synthetase activity Corynebacterium sp. ?
-
 ?
additional information uses 5,10-methylenen-5,6,7,8-tetrahydropteroylpolyglutamate as the preferred, and sometimes only polyglutamate substrate Bacteria ?
-
 ?
additional information monoglutamyl form and polyglutamyl forms of 5,6,7,8-tetrahydropteroate are the preferred substrates Cricetulus griseus ?
-
 ?
UTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Corynebacterium sp. UDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Lacticaseibacillus casei
monomer 1 * 53000, SDS-PAGE Corynebacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5 10
-
 Corynebacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
8 10 8: 20% of maximal activity, 9.5-10: maximal activity Corynebacterium sp.