Any feedback?
Literature summary for 6.3.2.13 extracted from
- Unusual substrate specificity of the peptidoglycan MurE ligase from Erysipelothrix rhusiopathiae (2016), Biochimie, 121, 209-218 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene murE, recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)/pLysS, subcloning in Escherichia coli strain DH5alpha | Erysipelothrix rhusiopathiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D399A | site-directed mutagenesis, inactive mutant | Erysipelothrix rhusiopathiae |
| D399N | site-directed mutagenesis, almost inactive mutant | Erysipelothrix rhusiopathiae |
| F340A | site-directed mutagenesis, almost inactive mutant | Erysipelothrix rhusiopathiae |
| F340Y | site-directed mutagenesis, the mutant shows reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme | Erysipelothrix rhusiopathiae |
| H398A | site-directed mutagenesis, the mutant shows reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme | Erysipelothrix rhusiopathiae |
| H398D | site-directed mutagenesis, the mutant shows highly reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme | Erysipelothrix rhusiopathiae |
| additional information | construction of an enzyme mutant MurEEr(HDNR/DNPR) with exchange of the HDNR morif to a DNPR motif, which is characteristic of meso-diaminopimelate-adding enzymes. The mutant shows increased activity with substrate meso-2,6-diaminoheptanedioate and reduced activity with substrate L-alanine | Erysipelothrix rhusiopathiae |
| N400A | site-directed mutagenesis, the mutant shows reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme | Erysipelothrix rhusiopathiae |
| N400P | site-directed mutagenesis, the mutant shows slightly reduced activity with substrate meso-2,6-diaminoheptanedioate, but increased activity with substrate L-alanine compared to wild-type enzyme | Erysipelothrix rhusiopathiae |
| R401A | site-directed mutagenesis, almost inactive mutant | Erysipelothrix rhusiopathiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
ATP | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae |  |
| 0.16 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 0.21 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-seryl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 0.23 | - |
UDP-N-acetyl-alpha-D-muramoyl-glycyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 1.8 | - |
L-alanine | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 10 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
| 64 | - |
L-alanine | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
| 77 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Erysipelothrix rhusiopathiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate | Erysipelothrix rhusiopathiae | - |
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Erysipelothrix rhusiopathiae | E7FUC9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzyme from Escherichia coli strain BL21(DE3)/pLysS by nickel affinity chromatography | Erysipelothrix rhusiopathiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UDP-N-acetyl-alpha-D-muramoyl-glycyl-D-glutamate + L-alanine | - |
Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-glycyl-gamma-D-glutamyl-L-alanine | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + (2S,6S)-2,6-diaminoheptanedioate | 7.8% activity with (2S,6S)-2,6-diaminoheptanedioate compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-(2S,6S)-2,6-diaminoheptanedioate | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + beta-chloro-L-alanine | 15% activity with beta-chloro-L-alanine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-beta-chloro-L-alanine | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + glycine | 7% activity with glycine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-glycine | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-alanine | highest activity with L-alanine compared to the other amino acids | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-alanine | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-diaminobutyrate | 7.9% activity with L-diaminobutyrate compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-diaminobutyrate | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-homoserine | 10% activity with L-homoserine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-homoserine | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-propargylglycine | 20% activity with L-propargylglycine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-propargylglycine | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-serine | 30% activity with L-serine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-serine | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-vinylglycine | 58% activity with L-vinylglycine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-vinylglycine | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate | - |
Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate | 30% activity with meso-2,6-diaminoheptanedioate compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate | - |
? | |
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-seryl-D-glutamate + L-alanine | - |
Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-seryl-gamma-D-glutamyl-L-alanine | - |
? | |
| additional information | enzyme substrate specificity, overview. Poor activity with D-alanine, L-lysine, L-ornithine, L-cysteine, L-allylglycine, L-1-aminoethylphosphonic acid, L-alanine amide, L-norvaline and L-alpha-aminobutyric acid. Enzyme-ligand interaction analysis, overview | Erysipelothrix rhusiopathiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Meso-diaminopimelate-adding enzyme | - |
Erysipelothrix rhusiopathiae |
| MurE | - |
Erysipelothrix rhusiopathiae |
| MurEEr | - |
Erysipelothrix rhusiopathiae |
| peptidoglycan MurE ligase | - |
Erysipelothrix rhusiopathiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Erysipelothrix rhusiopathiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.03 | - |
L-alanine | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
| 1.83 | - |
ATP | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 2.83 | - |
L-alanine | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 3.33 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 3.67 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-seryl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 4.17 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 4.83 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
| 8.83 | - |
UDP-N-acetyl-alpha-D-muramoyl-glycyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.6 | - |
assay at | Erysipelothrix rhusiopathiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Erysipelothrix rhusiopathiae | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the DNPR motif that is characteristic of meso-diaminopimelate-adding enzymes is replaced by HDNR | Erysipelothrix rhusiopathiae |
| additional information | the HDNR motif plays a role in the interaction of the enzyme with L-alanine and meso-diaminopimelic acid. ENzyme structure homology modeling | Erysipelothrix rhusiopathiae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
L-alanine | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
| 0.054 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 0.483 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
| 1.57 | - |
L-alanine | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 11.44 | - |
ATP | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 17.48 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-seryl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 20.81 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
| 38.39 | - |
UDP-N-acetyl-alpha-D-muramoyl-glycyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
