Literature summary for 6.3.2.13 extracted from

Paradis-Bleau, C.; Lloyd, A.; Sanschagrin, F.; Maaroufi, H.; Clarke, T.; Blewett, A.; Dowson, C.; Roper, D.I.; Bugg, T.D.; Levesque, R.C.
Pseudomonas aeruginosa MurE amide ligase: enzyme kinetics and peptide inhibitor (2009), Biochem. J., 421, 263-272.

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Inhibitors

Inhibitors	Comment	Organism	Structure
MurEP1	time-dependent inhibition	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.03	-	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate	-	Pseudomonas aeruginosa
0.14	-	meso-diaminopimelic acid	-	Pseudomonas aeruginosa
0.225	-	ATP	-	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	SDS-PAGE	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ATP + meso-diaminopimelic acid	assay at pH 8.0	Pseudomonas aeruginosa	ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid	-	?

Synonyms

Synonyms	Comment	Organism
amide ligase	-	Pseudomonas aeruginosa
MurE	-	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Pseudomonas aeruginosa

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.5	-	30 min pre-incupation	Pseudomonas aeruginosa	MurEP1
0.8	-	10 min pre-incubation	Pseudomonas aeruginosa	MurEP1

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Release 2023.1 (January 2023)