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Literature summary for 6.3.2.13 extracted from
- The MurE synthetase from Thermotoga maritima is endowed with an unusual D-lysine adding activity (2006), J. Biol. Chem., 281, 15680-15686.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strains BL21(DE3)/pLysS and DH5alpha | Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | at 68°C | Thermotoga maritima |  |
| 1.7 | - |
D-Lysine | at 68°C | Thermotoga maritima | |
| 2.8 | - |
L-lysine | at 68°C | Thermotoga maritima | |
| 3.6 | - |
ATP | at 68°C | Thermotoga maritima | |
| 4.8 | - |
meso-diaminopimelic acid | at 68°C | Thermotoga maritima | |
| 27 | - |
L-ornithine | at 68°C | Thermotoga maritima | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 15 mM for optimal activity | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
SDS-PAGE | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-nitrilotriacetate-agarose chromatography | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + D-lysine | - |
Thermotoga maritima | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-D-lysine | - |
? | |
| ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + D-lysine | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-D-lysine | - |
? | |
| ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine | - |
Thermotoga maritima | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine | - |
? | |
| ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine | - |
? | |
| ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-ornithine | - |
Thermotoga maritima | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-ornithine | - |
? | |
| ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-ornithine | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-ornithine | - |
? | |
| ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-diaminopimelic acid | - |
Thermotoga maritima | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid | - |
? | |
| ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-diaminopimelic acid | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MurE synthetase | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 68 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
the enzyme has 25% of its maximum activity at 37°C | Thermotoga maritima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9.4 | - |
- |
Thermotoga maritima |
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