Literature summary for 6.3.2.13 extracted from

Dementin, S.; Bouhss, A.; Auger, G.; Parquet, C.; Mengin-Lecreulx, D.; Dideberg, O.; van Heijenoort, J.; Blanot, D.
Evidence of a functional requirement for a carbamoylated lysine residue in MurD, MurE and MurF synthetases as established by chemical rescue experiments (2001), Eur. J. Biochem., 268, 5800-5807.

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Cloned(Commentary)

Cloned (Comment)	Organism
expresision of His-tagged MurE in Escherichia coli	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
Butylamine	500 mM, 88% inhibition	Escherichia coli
Butyrate	500 mM, 44% inhibition	Escherichia coli
ethylamine	500 mM, 73% inhibition	Escherichia coli
methylamine	500 mM, 80% inhibition	Escherichia coli
Propylamine	500 mM, 87% inhibition	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Escherichia coli	5737	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate	Escherichia coli	-	ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant MurE	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0047	-	K224A mutant	Escherichia coli
0.078	-	K224A mutant, in the presence of 500 mM propionate	Escherichia coli
1.24	-	recombinant wild-type MurE	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate	-	Escherichia coli	ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate	-	?

Synonyms

Synonyms	Comment	Organism
MurE	-	Escherichia coli

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Release 2023.1 (January 2023)