Any feedback?
Literature summary for 6.3.2.12 extracted from
- Characterization and inhibition of dihydrofolate synthetase from Neisseria gonorrhoeae (1984), Mol. Cell. Biochem., 59, 165-171.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 7,8-dihydrofolate | noncompetitive with respect to dihydropteroate, competitive with respect to L-Glu | Neisseria gonorrhoeae |  |
| p-hydroxymercuribenzoate | - |
Neisseria gonorrhoeae | |
| PCMB | - |
Neisseria gonorrhoeae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
7,8-dihydropteroate | - |
Neisseria gonorrhoeae | |
| 0.65 | - |
L-Glu | - |
Neisseria gonorrhoeae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | maximal activity at 80 mM | Neisseria gonorrhoeae | |
| K+ | monovalent cation requirement is satisfied by K+ or NH4+ | Neisseria gonorrhoeae | |
| Mg2+ | divalent cation required, Mg2+ or Mn2+ | Neisseria gonorrhoeae | |
| Mn2+ | divalent cation required | Neisseria gonorrhoeae | |
| NH4+ | monovalent cation required, K+ or NH4+, maximal activity at 20 mM | Neisseria gonorrhoeae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neisseria gonorrhoeae | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Neisseria gonorrhoeae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -60°C, stable | Neisseria gonorrhoeae |
| 4°C, 50% loss of activity after 6 weeks | Neisseria gonorrhoeae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 7,8-dihydropteroate + L-Glu | - |
Neisseria gonorrhoeae | ADP + phosphate + 7,8-dihydrofolate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.3 | - |
- |
Neisseria gonorrhoeae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | 8: about 25% of maximal activity, 9.5: about 85% of maximal activity | Neisseria gonorrhoeae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
