Literature summary for 6.3.1.9 extracted from

Henderson, G.B.; Yamaguchi, M.; Novoa, L.; Fairlamb, A.H.; Cerami, A.
Biosynthesis of the trypanosomatid metabolite trypanothione: purification and characterization of trypanothione synthetase from Crithidia fasciculata (1990), Biochemistry, 29, 3924-3929.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.007	-	N8-glutathionylspermidine	-	Crithidia fasciculata
0.02	-	N1-glutathionylspermidine	-	Crithidia fasciculata
0.4	-	MgATP2-	-	Crithidia fasciculata
0.914	-	gamma-L-Glu-L-Cys-Gly	-	Crithidia fasciculata
1.07	-	spermidine	-	Crithidia fasciculata

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	MgATP2- required	Crithidia fasciculata

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
81000	-	gel filtration	Crithidia fasciculata
87000	-	1 * 87000, SDS-PAGE	Crithidia fasciculata

Organism

Organism	UniProt	Comment	Textmining
Crithidia fasciculata	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Crithidia fasciculata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Crithidia fasciculata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP + H2O	-	Crithidia fasciculata	N1,N8-bis-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate + NH3	N1-glutathionylspermidine and N8-glutathionylspermidine are intermediates	?
glutathione + spermidine + ATP	ATP in form of MgATP2-	Crithidia fasciculata	glutathionylspermidine + ADP + phosphate	-	?
N8-glutathionylspermidine + glutathione + ATP	-	Crithidia fasciculata	N1,N8-bis(glutathionyl)spermidine + ADP + phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 87000, SDS-PAGE	Crithidia fasciculata

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
10	-	N1-glutathionylspermidine	-	Crithidia fasciculata
24.3	-	N8-glutathionylspermidine	-	Crithidia fasciculata
28.6	-	spermidine	-	Crithidia fasciculata

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	7.8	synthesis of both mono-glutathionylspermidine and di-glutathionylspermidine conjugates	Crithidia fasciculata

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Release 2023.1 (January 2023)