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Literature summary for 6.3.1.9 extracted from

  • Koch, O.; Cappel, D.; Nocker, M.; Jaeger, T.; Flohe, L.; Sotriffer, C.A.; Selzer, P.M.
    Molecular dynamics reveal binding mode of glutathionylspermidine by trypanothione synthetase (2013), PLoS ONE, 8, e56788.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.9

Inhibitors

Inhibitors Comment Organism Structure
prochlorperazine inhibitor DDD66604, binding mode and structure, overview Leishmania major

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ binding sites for N8-glutathionylspermidine , two Mg2+ ions, structure modeling, overview. For the second step of trypanothione synthesis glutathionylspermidine is bound in a way that preferentially allows N1-glutathionylation of N8-glutathionylspermidine, classifying N8-glutathionylspermidine as the favoured substrate Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 glutathione + spermidine + 2 ATP Leishmania major overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase, overview N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
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glutathione + spermidine + ATP Leishmania major reaction of EC 6.3.1.8, step one N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate
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N1-glutathionylspermidine + glutathione + ATP Leishmania major step two N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
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N8-glutathionylspermidine + glutathione + ATP Leishmania major step two, N8-glutathionylspermidine is the favoured substrate N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
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Organism

Organism UniProt Comment Textmining
Leishmania major
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + spermidine + 2 ATP overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase, overview Leishmania major N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
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 ?
glutathione + spermidine + ATP reaction of EC 6.3.1.8, step one Leishmania major N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate
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N1-glutathionylspermidine + glutathione + ATP step two Leishmania major N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
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N8-glutathionylspermidine + glutathione + ATP step two, N8-glutathionylspermidine is the favoured substrate Leishmania major N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
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Synonyms

Synonyms Comment Organism
Trypanothione synthetase
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 Leishmania major
TryS
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 Leishmania major

Cofactor

Cofactor Comment Organism Structure
ATP
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 Leishmania major

General Information

General Information Comment Organism
metabolism trypanothione synthetase catalyses the two-step biosynthesis of trypanothione from spermidine and glutathione Leishmania major
additional information binding sites for glutathione, ATP and two Mg2+ ions, molecular docking and molecular dynamics simulations, strructure modeling, detailed overview Leishmania major