Cloned (Comment) | Organism |
---|---|
gene TRYS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli | Leishmania major |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | substrate inhibition | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | hyperbolic kinetics, steady-state kinetics | Leishmania major | |
0.04 | - |
glutathionylspermidine | pH 7.7, 25°C, recombinant trypanothione synthase | Leishmania major | |
0.063 | - |
MgATP2- | pH 7.7, 25°C, recombinant trypanothione synthase | Leishmania major | |
0.089 | - |
glutathione | pH 7.7, 25°C, recombinant trypanothione synthase | Leishmania major | |
0.94 | - |
spermidine | pH 7.7, 25°C, recombinant trypanothione synthase | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Leishmania major | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | optimal up to 50 mM | Leishmania major | |
KCl | optimal up to 50 mM | Leishmania major | |
Mg2+ | as MgATP2- | Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 glutathione + spermidine + 2 ATP | Leishmania major | trypanothione synthase overall reaction | N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate | - |
? | |
glutathione + glutathionylspermidine + ATP | Leishmania major | trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | Leishmania major | trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 | glutathionylspermidine + ADP + phosphate | - |
? | |
additional information | Leishmania major | phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | Q711P7 | gene TRYS; gene TRYS | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, dialysis, and ion exchange chromatography | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 glutathione + spermidine + 2 ATP | trypanothione synthase overall reaction | Leishmania major | N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate | - |
? | |
glutathione + glutathionylspermidine + ATP | trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 | Leishmania major | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + glutathionylspermidine + ATP | trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 | Leishmania major | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 | Leishmania major | glutathionylspermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 | Leishmania major | glutathionylspermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | ATP in form of MgATP2- | Leishmania major | glutathionylspermidine + ADP + phosphate | - |
? | |
additional information | phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome | Leishmania major | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TryS | - |
Leishmania major |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Leishmania major |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
glutathione | pH 7.7, 25°C, recombinant trypanothione synthase | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
with substrates glutathionylspermidine and spermidine | Leishmania major |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | as MgATP2-, the enzyme is specific for ATP and shows only negligible activity with other NTPs | Leishmania major |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
glutathione | pH 7.7, 25°C, recombinant trypanothione synthase | Leishmania major |