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Literature summary for 6.3.1.9 extracted from

  • Oza, S.L.; Shaw, M.P.; Wyllie, S.; Fairlamb, A.H.
    Trypanothione biosynthesis in Leishmania major (2005), Mol. Biochem. Parasitol., 139, 107-116.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene TRYS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli Leishmania major

Inhibitors

Inhibitors Comment Organism Structure
glutathione substrate inhibition Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information hyperbolic kinetics, steady-state kinetics Leishmania major
0.04
-
glutathionylspermidine pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major
0.063
-
MgATP2- pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major
0.089
-
glutathione pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major
0.94
-
spermidine pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Leishmania major 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2SO4 optimal up to 50 mM Leishmania major
KCl optimal up to 50 mM Leishmania major
Mg2+ as MgATP2- Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 glutathione + spermidine + 2 ATP Leishmania major trypanothione synthase overall reaction N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
?
glutathione + glutathionylspermidine + ATP Leishmania major trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
glutathione + spermidine + ATP Leishmania major trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 glutathionylspermidine + ADP + phosphate
-
?
additional information Leishmania major phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome ?
-
?

Organism

Organism UniProt Comment Textmining
Leishmania major Q711P7 gene TRYS; gene TRYS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, dialysis, and ion exchange chromatography Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + spermidine + 2 ATP trypanothione synthase overall reaction Leishmania major N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
?
glutathione + glutathionylspermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 Leishmania major N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
glutathione + glutathionylspermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 Leishmania major N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
glutathione + spermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 Leishmania major glutathionylspermidine + ADP + phosphate
-
?
glutathione + spermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9 Leishmania major glutathionylspermidine + ADP + phosphate
-
?
glutathione + spermidine + ATP ATP in form of MgATP2- Leishmania major glutathionylspermidine + ADP + phosphate
-
?
additional information phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome Leishmania major ?
-
?

Synonyms

Synonyms Comment Organism
TryS
-
Leishmania major

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Leishmania major

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
glutathione pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
with substrates glutathionylspermidine and spermidine Leishmania major

Cofactor

Cofactor Comment Organism Structure
ATP as MgATP2-, the enzyme is specific for ATP and shows only negligible activity with other NTPs Leishmania major

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
glutathione pH 7.7, 25°C, recombinant trypanothione synthase Leishmania major