Literature summary for 6.3.1.9 extracted from

Oza, S.L.; Tetaud, E.; Ariyanayagam, M.R.; Warnon, S.S.; Fairlamb, A.H.
A Single Enzyme Catalyses Formation of Trypanothione from Glutathione and Spermidine in Trypanosoma cruzi (2002), J. Biol. Chem., 277, 35853-35861.

Search for more literature for 6.3.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and Saccharomyces cerevisiae	Trypanosoma cruzi

Inhibitors

Inhibitors	Comment	Organism	Structure
4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid	above 100 mM	Trypanosoma cruzi
ammonium sulfate	almost complete inhibition at 500 mM	Trypanosoma cruzi
KCl	approx. 50% inhibition at 500 mM	Trypanosoma cruzi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.053	-	ATP	pH 8.0	Trypanosoma cruzi
0.066	-	N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine	pH 8.0	Trypanosoma cruzi
0.19	-	GSH	pH 8.0, with N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine	Trypanosoma cruzi

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity	Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64000	-	gel filtration	Trypanosoma cruzi
73560	-	deduced from nucleotide sequence	Trypanosoma cruzi
73740	-	MALDI mass spectrometry	Trypanosoma cruzi
73744	-	1 * 73744, MALDI mass spectrometry	Trypanosoma cruzi
74500	-	SDS-PAGE	Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP	Trypanosoma cruzi	-	N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate	trivial name trypanothione	?

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	Q9GT49	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged TcTryS, Ni2+-affinity, anion exchange	Trypanosoma cruzi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.77	-	-	Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP	-	Trypanosoma cruzi	N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate	trivial name trypanothione	?
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP	multifunctional enzyme having also glutathionylspermidine synthetase, glutathionylaminopropylcadaverine synthetase, homotrypanothione synthetase and a weak amidase activity	Trypanosoma cruzi	N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate	trivial name trypanothione	?

Subunits

Subunits	Comment	Organism
monomer	1 * 73560, deduced from nucleotide sequence	Trypanosoma cruzi
monomer	1 * 73744, MALDI mass spectrometry	Trypanosoma cruzi
monomer	1 * 74500, SDS-PAGE	Trypanosoma cruzi

Synonyms

Synonyms	Comment	Organism
TcTryS	-	Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.8	-	N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine	pH 8.0	Trypanosoma cruzi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.1	-	-	Trypanosoma cruzi

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	10	approx. 50% of maximal activity at pH 6.6 and pH 9.5 respectively	Trypanosoma cruzi

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Release 2023.1 (January 2023)