Any feedback?
Literature summary for 6.3.1.8 extracted from
- Comparison of the functions of glutathionylspermidine synthetase/amidase from E. coli and its predicted homologues YgiC and YjfC (2012), Int. J. Biochem. Mol. Biol., 3, 302-312.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of enzyme mutant GspSA C59A in Escherichia coli strain BL21(DE3) | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + spermidine + ATP | Escherichia coli | - |
glutathionylspermidine + ADP + phosphate | - |
? | |
| additional information | Escherichia coli | Escherichia coli proteins YgiC and YjfC, encoded by genes ygiC and yjfC, cannot synthesize G-Sp, GSH, or GSH intermediates, they are no glutathionylspermidine synthetase/amidases, but show ATP-hydrolysis activity | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0AES0 | gene gss | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme mutant GspSA C59A from Escherichia coli strain BL21(DE3) | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + spermidine + ATP | - |
Escherichia coli | glutathionylspermidine + ADP + phosphate | - |
? | |
| additional information | Escherichia coli proteins YgiC and YjfC, encoded by genes ygiC and yjfC, cannot synthesize G-Sp, GSH, or GSH intermediates, they are no glutathionylspermidine synthetase/amidases, but show ATP-hydrolysis activity | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bifunctional glutathionylspermidine synthetase/amidase | UniProt | Escherichia coli |
| Glutathionylspermidine synthetase/amidase | - |
Escherichia coli |
| GspSA | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
purified recombinant GspSA C59A, 3 min, inactivation | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
