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Literature summary for 6.3.1.8 extracted from

  • Comini, M.; Menge, U.; Wissing, J.; Flohe, L.
    Trypanothione synthesis in Crithidia revisited (2005), J. Biol. Chem., 280, 6850-6860.
    View publication on PubMed
Search for more literature for 6.3.1.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 ATP pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction Crithidia fasciculata
1.175
-
 glutathione pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction Crithidia fasciculata
7.424
-
 spermidine pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction Crithidia fasciculata

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ as MgATP2- Crithidia fasciculata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + spermidine + ATP Crithidia fasciculata
-
 glutathionylspermidine + ADP + phosphate
-
 ?
glutathione + spermidine + ATP Crithidia fasciculata trypanothione synthase glutathionylspermidine + ADP + phosphate
-
 ?
additional information Crithidia fasciculata the trypanothione synthase also converts glutathionylspermidine further to trypanothione catalyzing the reaction of EC 6.3.1.9 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Crithidia fasciculata P90518
-
-
Crithidia fasciculata Q5DM89 trypanothione synthase; a dual specificity enzyme
-

Reaction

Reaction Comment Organism Reaction ID
glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate the reaction is catalyzed by trypanothione synthase and glutathionylspermidine synthase in Crithidia fasciculata Crithidia fasciculata
a
glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate the reaction is catalyzed by trypanothione synthase, EC 6.3.1.9, and glutathionylspermidine synthase, EC 6.3.1.8, in Crithidia fasciculata Crithidia fasciculata
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
 about, purified recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction Crithidia fasciculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + spermidine + 2 ATP trypanothione synthase overall reaction Crithidia fasciculata N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
 ?
glutathione + spermidine + ATP
-
 Crithidia fasciculata glutathionylspermidine + ADP + phosphate
-
 ?
glutathione + spermidine + ATP trypanothione synthase Crithidia fasciculata glutathionylspermidine + ADP + phosphate
-
 ?
glutathione + spermidine + ATP specific for, the enzyme is distinct from the trypanothione synthase, which also catalyzes the reaction but converts glutathionylsperimidine further to trypanothione Crithidia fasciculata glutathionylspermidine + ADP + phosphate
-
 ?
additional information the trypanothione synthase also converts glutathionylspermidine further to trypanothione catalyzing the reaction of EC 6.3.1.9 Crithidia fasciculata ?
-
 ?

Synonyms

Synonyms Comment Organism
GspS
-
 Crithidia fasciculata
TryS
-
 Crithidia fasciculata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Crithidia fasciculata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
 spermidine pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction Crithidia fasciculata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Crithidia fasciculata

Cofactor

Cofactor Comment Organism Structure
ATP as MgATP2- Crithidia fasciculata