Cloned (Comment) | Organism |
---|---|
expression of a recombinant histidine-tagged enzyme in Escherichia coli | Crithidia fasciculata |
Protein Variants | Comment | Organism |
---|---|---|
C79A | mutant retains synthetase activity, but loses amidase activity | Crithidia fasciculata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
spermidine | pH 7.3, 25°C, synthetase activity | Crithidia fasciculata | |
0.078 | - |
spermidine | pH 7.3, 25°C, synthetase activity of the C79A mutant | Crithidia fasciculata | |
0.096 | - |
MgADP- | pH 7.3, 25°C, synthetase activity of the C79A mutant | Crithidia fasciculata | |
0.114 | - |
MgADP- | pH 7.3, 25°C, synthetase activity | Crithidia fasciculata | |
0.171 | - |
glutathione | pH 7.3, 25°C, synthetase activity of the C79A mutant | Crithidia fasciculata | |
0.242 | - |
glutathione | pH 7.3, 25°C, synthetase activity | Crithidia fasciculata | |
0.5 | - |
glutathionylspermidine | pH 7.3, 25°C, amidase activity | Crithidia fasciculata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80520 | - |
MALDI-TOF MS | Crithidia fasciculata |
85200 | - |
gel filtration | Crithidia fasciculata |
86000 | - |
1 * 86000, SDS-PAGE | Crithidia fasciculata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | P90518 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-chelating and anion-exchange chromatography | Crithidia fasciculata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.9 | - |
pH 7.3, 25°C, synthetase activity | Crithidia fasciculata |
Storage Stability | Organism |
---|---|
-80°C, 4 months, loses less than 10% synthetase activity | Crithidia fasciculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gamma-L-Glu-L-Cys-Gly + spermidine + MgATP2- | - |
Crithidia fasciculata | N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + MgADP- + phosphate | - |
? | |
glutathionylspermidine + H2O | amidase activity | Crithidia fasciculata | glutathione + spermidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 86000, SDS-PAGE | Crithidia fasciculata |
Synonyms | Comment | Organism |
---|---|---|
GspS | - |
Crithidia fasciculata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.31 | - |
- |
Crithidia fasciculata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8 | - |
Crithidia fasciculata |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Crithidia fasciculata | isoelectric focusing | 4.7 | 4.6 |