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Literature summary for 6.3.1.8 extracted from

  • Oza, S.L.; Ariyanayagam, M.R.; Fairlamb, A.H.
    Characterization of recombinant glutathionylspermidine synthetase/amidase from Crithidia fasciculata (2002), Biochem. J., 364, 679-686.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of a recombinant histidine-tagged enzyme in Escherichia coli Crithidia fasciculata

Protein Variants

Protein Variants Comment Organism
C79A mutant retains synthetase activity, but loses amidase activity Crithidia fasciculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.059
-
spermidine pH 7.3, 25°C, synthetase activity Crithidia fasciculata
0.078
-
spermidine pH 7.3, 25°C, synthetase activity of the C79A mutant Crithidia fasciculata
0.096
-
MgADP- pH 7.3, 25°C, synthetase activity of the C79A mutant Crithidia fasciculata
0.114
-
MgADP- pH 7.3, 25°C, synthetase activity Crithidia fasciculata
0.171
-
glutathione pH 7.3, 25°C, synthetase activity of the C79A mutant Crithidia fasciculata
0.242
-
glutathione pH 7.3, 25°C, synthetase activity Crithidia fasciculata
0.5
-
glutathionylspermidine pH 7.3, 25°C, amidase activity Crithidia fasciculata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80520
-
MALDI-TOF MS Crithidia fasciculata
85200
-
gel filtration Crithidia fasciculata
86000
-
1 * 86000, SDS-PAGE Crithidia fasciculata

Organism

Organism UniProt Comment Textmining
Crithidia fasciculata P90518
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-chelating and anion-exchange chromatography Crithidia fasciculata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.9
-
pH 7.3, 25°C, synthetase activity Crithidia fasciculata

Storage Stability

Storage Stability Organism
-80°C, 4 months, loses less than 10% synthetase activity Crithidia fasciculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gamma-L-Glu-L-Cys-Gly + spermidine + MgATP2-
-
Crithidia fasciculata N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + MgADP- + phosphate
-
?
glutathionylspermidine + H2O amidase activity Crithidia fasciculata glutathione + spermidine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 86000, SDS-PAGE Crithidia fasciculata

Synonyms

Synonyms Comment Organism
GspS
-
Crithidia fasciculata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.31
-
-
Crithidia fasciculata

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8
-
Crithidia fasciculata

pI Value

Organism Comment pI Value Maximum pI Value
Crithidia fasciculata isoelectric focusing 4.7 4.6