Crystallization (Comment) | Organism |
---|---|
enzyme in complex with ATP, ATP-Tl+, ATP-Mn2+, or NAD-adenylate, hanging-drop vapour diffusion method, 15 mg/ml protein, 5 mM ATP, 20 mM NAD+ in 0.1 M sodium acetate, pH 5.2, 22% PEG 400, and 50 mM MgCl2, thallium acetate, or MnCl2 20°C, 24 h before data collection the crystale are soaked in a cryoprotectant buffer, X-ray diffraction structure determination at 1.3 A resolution, structure analysis and modeling | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required, 2 binding sites near the bound diphosphate at the ATP-binding site, can be substituted by Tl+ | Bacillus subtilis | |
Mg2+ | 2 Mg2+ ligands per molecule of enzyme | Bacillus subtilis | |
Tl+ | can substitute for K+ | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + NH3 | Bacillus subtilis | NAD-adenylate intermediate, key two step reaction in NAD+ biosynthesis | AMP + diphosphate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P08164 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+ | reaction mechanism, determination of deamido-NAD+-binding site, Asp173 is the key residue in both deprotonation of the primarily bound ammonium ion, and stabilization of the tetrahedral transition-state intermediate | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + NH3 | NAD-adenylate intermediate, two step reaction | Bacillus subtilis | AMP + diphosphate + NAD+ | - |
? | |
ATP + deamido-NAD+ + NH3 | NAD-adenylate intermediate, key two step reaction in NAD+ biosynthesis | Bacillus subtilis | AMP + diphosphate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer, crystal structure determination | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
NAD synthase | - |
Bacillus subtilis |
NAD synthetase | - |
Bacillus subtilis |
Nicotinamide adenine dinucleotide synthetase | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binuclear magnesium cluster | Bacillus subtilis |