Literature summary for 6.3.1.20 extracted from

Morris, T.W.; Reed, K.E.; Cronan, J.E., Jr.
Identification of the gene encoding lipoate-protein ligase A of Escherichia coli. Molecular cloning and characterization of the lplA gene and gene product (1994), J. Biol. Chem., 269, 16091-16100.

Search for more literature for 6.3.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme null mutant, normal transport of lipoic acid, but severe defect in incorporation and utilization of exogenously supplied lipoic acid and lipoic acid analogues. Strain is highly resistant to selenolipoate	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	x * 38000, calculated	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P32099	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
11.5	-	37°C	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + DL-lipoic acid + protein	-	Escherichia coli	AMP + diphosphate + DL-lipoyl-protein	-	?

Subunits

Subunits	Comment	Organism
?	x * 38000, calculated	Escherichia coli

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Release 2023.1 (January 2023)