Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.1.2 extracted from

  • Singh, K.K.; Ghosh, S.
    Regulation of glutamine synthetase isoforms in two differentially drought-tolerant rice (Oryza sativa L.) cultivars under water deficit conditions (2013), Plant Cell Rep., 32, 183-193.
    View publication on PubMed
Search for more literature for 6.3.1.2

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isozyme GS2 Oryza sativa 9507
-
cytosol isozyme OsGS1;1 Oryza sativa Japonica Group 5829
-
cytosol isozyme OsGS1;2 Oryza sativa 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Oryza sativa
Mg2+ required Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + NH3 Oryza sativa
-
 ADP + phosphate + L-glutamine
-
 ?
ATP + L-glutamate + NH3 Oryza sativa Japonica Group
-
 ADP + phosphate + L-glutamine
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 var. indica, drought-sensitive IR-64 cultivar and drought-tolerant Khitish cultivar, gene OsGS1;2
-
Oryza sativa P14655 OsGS2; var. indica, drought-sensitive IR-64 cultivar and drought-tolerant Khitish cultivar, gene OsGS2
-
Oryza sativa Japonica Group P14656 OsGS1;1; var. indica, drought-sensitive IR-64 cultivar and drought-tolerant Khitish cultivar, gene OsGS1;1
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa
-
leaf
-
 Oryza sativa Japonica Group
-
additional information mRNA and protein contents of GS isoforms, overview Oryza sativa
-
additional information mRNA and protein contents of GS isoforms, overview Oryza sativa Japonica Group
-
root
-
 Oryza sativa
-
root
-
 Oryza sativa Japonica Group
-
seedling
-
 Oryza sativa
-
seedling
-
 Oryza sativa Japonica Group
-
stem
-
 Oryza sativa
-
stem
-
 Oryza sativa Japonica Group
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 total GS, GS1 and GS2 activities in leaf, stem and root of rice in cultivars IR-64 and Khitish seedlings at various stages of water deprivation, overview Oryza sativa
additional information
-
 total GS, GS1 and GS2 activities in leaf, stem and root of rice in cultivars IR-64 and Khitish seedlings at various stages of water deprivation, overview Oryza sativa Japonica Group

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + NH3
-
 Oryza sativa ADP + phosphate + L-glutamine
-
 ?
ATP + L-glutamate + NH3
-
 Oryza sativa Japonica Group ADP + phosphate + L-glutamine
-
 ?
hydroxylamine + L-glutamine + ATP gamma-glutamylhydroxamate synthetase activity Oryza sativa L-gamma-glutamyl-hydroxamate + ammonia + ADP
-
 ?
hydroxylamine + L-glutamine + ATP gamma-glutamylhydroxamate synthetase activity Oryza sativa Japonica Group L-gamma-glutamyl-hydroxamate + ammonia + ADP
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oryza sativa
37
-
 assay at Oryza sativa Japonica Group

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Oryza sativa
7.5
-
 assay at Oryza sativa Japonica Group

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Oryza sativa
ATP
-
 Oryza sativa Japonica Group

Expression

Organism Comment Expression
Oryza sativa Japonica Group under drought stress, total GS activity in root and leaf decreases significantly in IR-64 seedlings in comparison to Khitish seedlings. The reduced GS activity in IR-64 leaf is mainly due to decrease in isozyme GS2 activity down
Oryza sativa under drought stress, total GS activity in root and leaf decreases significantly in IR-64 seedlings in comparison to Khitish seedlings. The reduced GS activity in IR-64 leaf is mainly due to decrease in isozyme GS2 activity, which correlates with decrease in corresponding transcript and polypeptide contents down
Oryza sativa total enzyme activity in stem of both the varieties is insensitive to drought additional information
Oryza sativa Japonica Group total enzyme activity in stem of both the varieties is insensitive to drought additional information
Oryza sativa gene GS1 transcript and polypeptide accumulate in leaf during drought, but GS1 activity is maintained at a constant level up
Oryza sativa Japonica Group gene GS1 transcript and polypeptide accumulate in leaf during drought, but GS1 activity is maintained at a constant level up

General Information

General Information Comment Organism
physiological function the enzyme catalyzes the ATP-dependent assimilation of ammonium into glutamate to yield glutamine, which is then used for the biosynthesis of essentially all nitrogenous compounds. Effect of water deprivation varies with variety, degree and duration of stress Oryza sativa
physiological function the enzyme catalyzes the ATP-dependent assimilation of ammonium into glutamate to yield glutamine, which is then used for the biosynthesis of essentially all nitrogenous compounds. Gene OsGS1;1 expression is differently regulated by drought stresss in the two rice Oryza sativa varieties, effect of water deprivation varies with variety, degree and duration of stress Oryza sativa Japonica Group