Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nitrogen sensor protein GlnK1 | the nitrogen sensor protein GlnK1 of the methanogenic archaeon interacts and forms stable complexes with glutamine synthetase GlnA1. Complex formation with GlnK1 in the absence of metabolites inhibits the activity of GlnA1. The nitrogen sensor GlnK1 allows finetuning control of the glutamine synthetase activity under changing nitrogen availabilities. The following model is proposed: under nitrogen limitation, increasing concentrations of 2-oxoglutarate stimulate maximal GlnA1 activity and transform GlnA1 into an activated conformation, which prevents inhibition by GlnK1. Upon a shift to nitrogen sufficiency after a period of nitrogen limitation, GlnA1 activity is reduced by decreasing internal 2-oxoglutarate concentrations through diminished direct activation and by GlnK1 inhibition | Methanosarcina mazei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | directly stimulates activity | Methanosarcina mazei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, calculated from sequence | Methanosarcina mazei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina mazei | Q8PY99 | - |
- |
Methanosarcina mazei DSM 3647 | Q8PY99 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina mazei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH3 | - |
Methanosarcina mazei | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | - |
Methanosarcina mazei DSM 3647 | ADP + phosphate + L-glutamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, calculated from sequence | Methanosarcina mazei |
Synonyms | Comment | Organism |
---|---|---|
GlnA1 | - |
Methanosarcina mazei |
MM_0964 | locus name | Methanosarcina mazei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanosarcina mazei |